Protocols developed

Given below is a list of protocols developed by the participating centres of PhytoFuRa. Click on the view link to see details about the protocol.

  1. CCRI, Nagpur - Development of SCAR markers for species-specific detection and identification of Phytophthora nicotianae
  2. CCRI, Nagpur - Development of Phytophthora nicotianae specific primers from extracellular cystatin-like cysteine protease inhibitor (EPIC1) gene
  3. CCRI, Nagpur - Detection of Phytophthora nicotianae in irrigation water by Ypt1 gene based nested PCR
  4. CCRI, Nagpur - Multiplex PCR asaay for simultaneous detection of two Phytophthora pathogens, P. nicotianae and P. palmivora
  5. CCRI, Nagpur - Development of conventional and Real-Time PCR assays for rapid and sensitive detection of P. nicotianae causing root rot and gummosis of citrus
  6. CPRI,Shimla - Dection of Phytophthora infestans using SCAR marker.
  7. CPRI,Shimla - Simultaneous detection of early and late blight of potato.
  8. CPRI,Shimla - Simultaneous detection of Fusarium spp and Phytophthora infestans
  9. CPRI,Shimla - Detection and quantification of P. infestans in host tissues using PCR protocols
  10. CPRI,Shimla - Development of ELISA based diagnosis for P. infestans
  11. CPRI,Shimla - Development of P. infestans Pol gene RNAi construct
  12. CTCRI,TVM - Rapid and sensitive detection of Phytophthora colocasiae responsible for the taro leaf blight using conventional and real-time PCR assay
  13. CTCRI,TVM - A rapid and efficient method for in vitro production of zoospores
  14. CTCRI,TVM - A rapid and efficient method for in vitro screening of taro for leaf blight disease caused by Phytophthora colocasiae
  15. CTCRI,TVM - A Simple and fficient Protocol for Rapid Regeneration and Propagation of Taro (Colocasia esculenta (L.) Schott.) in vitro from Apical Meristems
  16. CTCRI,TVM - AFLP fingerprinting of P. colocasiae
  17. CTCRI,TVM - Total RNA isolation from taro/P. colocasiae
  18. ICAR RC NEH, Umiam - A new approach for PCR based rapid detection of Ralstonia solanacearum from infected plants
  19. IARI, New DelhI - Detection of Polyketides producing Bacillus spp. by Multiplex Polymerase Chain Reaction (PCR)
  20. IARI, New DelhI - Development and application of Loop-Mediated Isothermal Amplification assay for detection of Ralstonia solanacearum- wilt pathogen of tomato plant
  21. IARI, New DelhI - Development of bioformulation of Pseudomonas fluorescens, Bacillus subtilis and Bacillus amyloliquefaciens
  22. IARI, New DelhI - Detection of Ralstonia solanacearum from Asymptomatic Tomato Plants, Irrigation Water, and Soil Through Non-selective Enrichment Medium with hrp Gene-Based Bio-PCR
  23. IIOR, Hyderabad - Screening technique against Phytophthora nicotianae
  24. IIOR, Hyderabad - Development of SCAR marker for specific detection of Fusarium oxysporrum f. sp carthami
  25. IIOR, Hyderabad - Identification of SSR markers linked to Fusarium wilt resistance
  26. IIVR-Varanasi - Molecular race identification of Fusarium oxysporum f.sp. lycopersici infecting tomato in India
  27. IIVR-Varanasi - Chitin synthase, a candidate gene for the molecular characterization of Fusarium isolates alternative to ITS region
  28. IIVR-Varanasi - Molecular characterization of Fusarium oxysporum f.sp. lycopersici isolates infecting tomato using polygalacturonase gene
  29. RRI, Kottayam - Application of DArT markers (SNP and silico DArT) for generating high density genetic linkage map in rubber (Hevea brasiliensis)
  30. YSPUHF,RC Kullu - Development of protocol/ new method for isolation of Phytophthora cactorum causing collar rot of apple from soil