Phytophthora

  1. ICAR-IISR, Kozhikode
  2. ICAR-CPRI, Shimla
  3. ICAR-CPCRI, Kasaragod
  4. ICAR-CTCRI, Trivandrum
  5. ICAR-NRC Citrus, Nagpur
  6. ICAR RC NEH, Umiam
  7. ICAR-YSPUHF,RC Kullu
1. ICAR-IISR, Kozhikode

S. No. Activity 2008-09 2009-10 2010-11 2011-12 Work done by
IVIIIIIIIVIIIIIIIVI IIIIIIV
Recruitment of Staff MA, SJE
Purchase of Equipment MA, SJE, JKG, KNB
01 Collection, maintenance and DNA isolation of P. capsici and nematodes MA, RSB, SJE
Study diversity of new P. capsici isolates through the use of biological markers (mating type, Metalaxyl sensitivity and virulence) MA, RSB
Genetic diversity and Fingerprinting of P. capsici and nematiode populations MA, SJE
Detection and quantification of P. capsici in host tissue MA, RSB, SJE
Development of detection kits MA, SJE
02 Species specific/strain specific detection and quantification in host tissue and soil SJE, RSB MA
03 Host- pathogen- environment interaction SJE, RSB
Pathogen- rhizosphere micro flora interaction SJE, RSB
04 Identification of AVR genes KNBMA
Markers for virulence and fungicide sensitivity RSB, MA
Identification and cloning of resistance genes, Trascription factors JKG, KNBMA
05 Screening of parental lines RSB SJE MA
Development of molecular markers for screening KNB RSBMA SJE
Raising of segregating mapping populations and molecular profiling MA, RSB, SJE
Construction of linkage map KNB SJE MA
Identification of QTLs and MAS KNB MA RSB, SJE
06 Seed/planting material/soil health management RSB, SJE
Plant health management RSB, SJE
Bio-control and IDM RSB, SJE
Mass multiplication and formulation development and evaluation RSB SJE
Bioinformatics Updating Phytophthora database SJE MA
Developing bioinformatics support system SJE MA
Developing PHYTOWEB portal for database creation and monitoring SJE MA

MA – M. Anandaraj; KNB – K. Nirmal Babu; JKG – Johnson K George; RSB – R. Suseela Bhai; SJE – Santhosh J Eapen

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2.ICAR-CPRI, Shimla

S. No. Activity 2008-09 2009-10 2010-11 2011-12 Work done by
IVIIIIIIIVIIIIIIIVI IIIIIIV
Recruitment of Staff
01 Collection, maintenance and DNA isolation of P. infestans MNB
Phenotypic differentiation of P. infestans isolates using biological markers MNB & BPS
Genotypic differentiation and fingerprinting of P. infestans using SSR markers MNB, BPS, DP & SKK
Mt DNA haplotype analysis for lineage determination BPS & MNB
Detection and quantification of P. infestans in host tissue DP, BPS & MNB
03 Development of inverted repeat RNAi gene construct of RXLR and CRN effector genes of P. infestans DP
Transformation of P. infestans with inverted repeat RNAi gene constructs of RXLR and CRN for development of knockout mutants DP
Characterization of P. infestans mutants for virulence, host range etc. DP
Development of host resistance by introduction of inverted repeat gene constructs into susceptible Indian potato cultivars. DP, BPS & MNB
04 Validation of molecular markers Acquisition of molecular markers tightly linked to R-genes and synthesis of PCR primers SKK
Validation of PCR markers in late blight differentials SKK
Screening of parental lines and late blight resistant commercial cultivars, selection of parents and hybridization SKK, BPS & MNB
Molecular mapping of quantitative trait loci (QTL) for horizontal resistance in mapping population Validation of available SSR markers, DNA isolation and genotyping using SSR markers SKK
Conversion of SSR molecular marker data into linkage map using Join Map software SKK
Correlation of LB Phenotypic data with molecular map to identify the QTLs SKK
05 Isolation of novel microorganism with activity against P. infestans BPS & MNB
Extraction and identification of microbial metabolites with activity against P. infestans MAK, MNB & BPS
06 Collection of weather and disease data under different spray schedules using popular varieties BPS & MNB
Development of yield loss assessment models BPS & MNB
Development of Decision Support System BPS & MNB
Study effect of elevated temperature on host resistance, fungicide efficacy and aggression of P. infestans BPS & MNB

BPS - B P Singh; MNB - M Narayana Bhat; DP - Debasis Patatnayak; SKC - S K Chakrabarti; SKK - S K Kaushik; MAK - M. A. Khan

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3. ICAR-CPCRI, Kasaragod

S. No. Activity 2008-09 2009-10 2010-11 2011-12 Work done by
IVIIIIIIIVIIIIIIIVI IIIIIIV
01Collection of isolates of Phytophthora sp. causing diseases of coconut and cocoa from disease endemic areas, characterization, documentation and conservation. (Cultural and morphological characters, identification and pathogenic variability). RC KD NR
Studies on mating types and its geographical distribution RC KD NR
RAPD analysis and SSR analysis to find out the diversity. Molecular characterization of Phytopthora isolates based on ITS region of rDNA. RC KD NR
02 Species specific/strain specific detection in host tissue and soil. Development of species – specific ITS primers for rapid detection of Phytophthora RC KD NR
Development of diagnostic kit for quick detection of the pathogen in soil and host tissue RC KD NR
03 Effect of environmental factors on Phytophthora diseases, Source of the inoculum and effect of different cultural practices on Phytophthora population in soil. RC KD NR
Effect of environmental factors on Phytophthora diseases, Source of the inoculum and effect of different cultural practices on Phytophthora population in soil. RC KD NR
Developing disease forecasting systems with the help of prediction models. RC KD NR
06 Seed / planting material and soil health management. RC KD NR
Integrated disease management involving cultural, biological and chemical control RC KD NR
Analysis of data and preparation of final report RC KD NR

RC – R . Chandramohan; KD – K. Devakumar; N. Ravikumar

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4.ICAR-CTCRI, Trivandrum

S. No. Activity 2009-10 2010-11 2011-12 Work done by
IIIIIIIVIIIIIIIVI IIIIIIV
01Collection of isolates of the pathogen from different places and purification RSM, VH & MLJ
Morphological and cultural characterization of the pathogen isolates RSM, VH & MLJ
AFLP analysis of P. colocasiae isolates RSM, VH & MLJ
AFLP and SSR analysis of taro genotypes RSM, VH & MLJ
02 Standardize the Real time PCR method for detection and quantification of pathogen propagules in planting material RSM, VH & MLJ
Field validation of molecular detection techniques like PCR for detection of pathogen in taro RSM, VH & MLJ
03 Observation on field trials and recording the epidemiological factors VH & MLJ
04 Identification of suitable promoter for elicitor gene isolated from P. colocasiae RSM, VH & MLJ
05 Standardization of use of elicitor protein isolated from P. colocasiae for in vitro screening of taro genotypes against leaf blight RSM, VH & MLJ
Development of in vitro screening method for Colocasia using specific elicitor protein RSM
Mapping of taro genotypes to leaf blight RSM, VH & MLJ
06 Preparation of bioformulation from the existing bio control agents against leaf blight RSM, VH & MLJ
Evaluation of suitable bioformulation technique in glass house RSM, VH & MLJ
Selection of effective bioformulation for control of tuber rot and leaf blight. VH & MLJ
Lay out field trial with bio-control formulation and IDM for management of leaf blight. VH & MLJ
Observation and recording the data on disease incidence in the IDM experiments RSM, VH & MLJ
On farm demonstration of IDM technology RSM, VH & MLJ
Observation of performance of IDM technology RSM, VH & MLJ
Indexing of planting material of taro for pathogen through PCR and ELISA RSM
Development of healthy planting material RSM
Multiplication of healthy planting materials RSM, VH & MLJ
Observation on performance of indexed planting material of taro at farmers’ field RSM, VH & MLJ
Data analysis and report writing RSM, VH & MLJ

RSM - R. S. Misra; VH - Vinayaka Hegde; MLJ - M. L. Jeeva

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5. ICAR-NRC Citrus, Nagpur

S. No. Activity 2009-10 2010-11 2011-12 Work done by
IIIIIIIVIIIIIIIVI IIIIIIV
Procurement of equipments
Recruitment of SRFs
01 Collection and conservation of Phytophthora spp. isolates AKD
Diversity analysis AKD
02 Development of molecular diagnostic kit, quantification in host tissue and soil, large scale indexing AKD
03 Host-pathogen and pathogen-pathogen interaction studies AKD & VB
04 Host Resistance : Screening of citrus rootstocks to evaluate resistance against Phytophthora spp. AKD & IPS
05 Management : Search for novel bioagents and testing promising bioagents against Phytophthora spp AKD

AKD - A.K. Das; VB - V. Bamel; IPS - I. P. Singh

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6.ICAR RC NEH, Umiam

S. No. Activity 2008-09 2009-10 2010-11 2011-12 Work done by
IVIIIIIIIVIIIIIIIVI IIIIIIV
Extensive literature search RD/PB
01 Survey of Phytophthora in citrus prone in disease prone area of different agro-climatic zone. RD/PB
Isolation and characterization of Phytophthora spp. from plants as well as soil. RD/PB
Characterization Phytophthora isolates using PCR based molecular tool. RD/PB
02 Isolation and characterization of antagonistic microbes RD/PB
Test the pathogenicity of collected isolates of Phytophthora spp. RD/PB
Test the bio-efficacy of bioagent against Phytophthora spp. under in vitro and protected conditions. RD/PB
Formulation studies and field evaluation of selected bioagents RD/PB
03 Evaluation of available ITKs RD/PB
Conduct training to agriculture officers and extension worker on diagnosis and management of citrus diseases RD/PB
Submission of final report RD/PB

RD - R. Dutta; PB - P. Baiswar

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7.ICAR-YSPUHF,RC Kullu

S. No. Activity 2008-09 2009-10 2010-11 2011-12 Work done by
IVIIIIIIIVIIIIIIIVI IIIIIIV
Recruitment of staff (SRF) IMS, DG, PG VS, BG, RK
Procurement of Equipments IMS, DG, PG VS, BG, RK
01 Survey for the collection of diseased samples IMS, DG, PG VS, BG, RK
Collection of soil samples from wilted and healthy plants IMS, DG, PG VS, BG, RK
Isolation of pathogen from wilted disease samples IMS, DG, PG VS, BG, RK
Estimation of total microbial population (Fungi, Bacteria and Actinomycetes) and nematode fauna form soil samples of healthy and wilted guava plants. IMS, DG, PG VS, BG, RK
Diversity analysis using phenotypic methods and other taxonomic methods IMS, DG, PG VS, BG, RK
Deposition of Fusarium isolates at NBAIM, Mau IMS, DG, PG VS, BG, RK
02Standardization of DNA isolation protocol of Fusarium isolates IMS, DG, PG VS, BG, RK
Screening of RAPD and STMS markers IMS, DG, PG VS, BG, RK
Identification of repeatable differentiating bands IMS, DG, PG VS, BG, RK
Elution of repeatable band IMS, DG, PG VS, BG, RK
Cloning of suitable vector (pGEMT / any T vector) IMS, DG, PG VS, BG, RK
Screening in M-13 phage vector IMS, DG, PG VS, BG, RK
Sequencing IMS, DG, PG VS, BG, RK
Designing of primers IMS, DG, PG VS, BG, RK
Validation of results IMS, DG, PG VS, BG, RK
Development of PCR based diagnostic kit IMS, DG, PG VS, BG, RK
03 Weather data will be recoded weekly with the help of automatic weather station for its correlation with wilt incidence and development of prediction model IMS, DG, PG VS, BG, RK
Collection weather data IMS, DG, PG VS, BG, RK
Soil data will be recoded from healthy and wilted guava plants from hot spot (Major guava growing belt at Allahabad and Kanpur) IMS, DG, PG VS, BG, RK
Estimation of total microbial population Isolation of frequently occuring / domination fungi / bacteria and its screening against virulent Fusarium spp. isolates for its synergistic / antagonistic efficacy. IMS, DG, PG VS, BG, RK
Development of prediction models IMS, DG, PG VS, BG, RK
03Host Resistance Inoculation of Fusarium isolates to guava germ plasma using stem hole inoculation technique and observation on wilt incidence IMS, DG, PG VS, BG, RK
Identification of wilt resistant source IMS, DG, PG VS, BG, RK
Compilation of data, statistical analysis and compilation of report and submission of final report to New work Coordinator IMS, DG, PG VS, BG, RK
04 Development of Integrated diseases management schedule (Field condition)
• Evaluation of various co crops / intercrop (Ginger, Asperagus and Serpagandha)
• Application of different Trichoderma harzianium, Trichoderma viride and Aspergiluus niger (Procured from PDBC, Bangalore)
• Use of antagonistic crop (Marigold, Garlic and Turmeric)
• Use of organic manure (Application of cow pat pit, Jeevamrit, Amritpani and Bijamrit)
IMS, DG, PG VS, BG, RK

IMS - I.M. Sharma; DG - D. Gupta; PG - P. Gupta; VS - Vinay Sagar; BG - Bhupesh Gupta; RK - Rajinder Kaur

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