Phytophthora

1. ICAR-IISR, Kozhikode
2. ICAR-CPRI, Shimla
3. ICAR-CPCRI, Kasaragod
4. ICAR-CTCRI, Trivandrum
5. ICAR-NRC Citrus, Nagpur
6. ICAR RC NEH, Umiam
7. ICAR-YSPUHF,RC Kullu

1. ICAR-IISR, Kozhikode

S. No.	Activity	2008-09	2009-10				2010-11				2011-12				Work done by
		IV	I	II	III	IV	I	II	III	IV	I	II	III	IV
	Recruitment of Staff														MA, SJE
	Purchase of Equipment														MA, SJE, JKG, KNB
01	Collection, maintenance and DNA isolation of P. capsici and nematodes														MA, RSB, SJE
	Study diversity of new P. capsici isolates through the use of biological markers (mating type, Metalaxyl sensitivity and virulence)														MA, RSB
	Genetic diversity and Fingerprinting of P. capsici and nematiode populations														MA, SJE
	Detection and quantification of P. capsici in host tissue														MA, RSB, SJE
	Development of detection kits														MA, SJE
02	Species specific/strain specific detection and quantification in host tissue and soil														SJE, RSB MA
03	Host- pathogen- environment interaction														SJE, RSB
	Pathogen- rhizosphere micro flora interaction														SJE, RSB
04	Identification of AVR genes														KNBMA
	Markers for virulence and fungicide sensitivity														RSB, MA
	Identification and cloning of resistance genes, Trascription factors														JKG, KNBMA
05	Screening of parental lines														RSB SJE MA
	Development of molecular markers for screening														KNB RSBMA SJE
	Raising of segregating mapping populations and molecular profiling														MA, RSB, SJE
	Construction of linkage map														KNB SJE MA
	Identification of QTLs and MAS														KNB MA RSB, SJE
06	Seed/planting material/soil health management														RSB, SJE
	Plant health management														RSB, SJE
	Bio-control and IDM														RSB, SJE
	Mass multiplication and formulation development and evaluation														RSB SJE
	Bioinformatics Updating Phytophthora database														SJE MA
	Developing bioinformatics support system														SJE MA
	Developing PHYTOWEB portal for database creation and monitoring														SJE MA

MA – M. Anandaraj; KNB – K. Nirmal Babu; JKG – Johnson K George; RSB – R. Suseela Bhai; SJE – Santhosh J Eapen

2.ICAR-CPRI, Shimla

S. No.	Activity	2008-09	2009-10				2010-11				2011-12				Work done by
		IV	I	II	III	IV	I	II	III	IV	I	II	III	IV
	Recruitment of Staff
01	Collection, maintenance and DNA isolation of P. infestans														MNB
	Phenotypic differentiation of P. infestans isolates using biological markers														MNB & BPS
	Genotypic differentiation and fingerprinting of P. infestans using SSR markers														MNB, BPS, DP & SKK
	Mt DNA haplotype analysis for lineage determination														BPS & MNB
	Detection and quantification of P. infestans in host tissue														DP, BPS & MNB
03	Development of inverted repeat RNAi gene construct of RXLR and CRN effector genes of P. infestans														DP
	Transformation of P. infestans with inverted repeat RNAi gene constructs of RXLR and CRN for development of knockout mutants														DP
	Characterization of P. infestans mutants for virulence, host range etc.														DP
	Development of host resistance by introduction of inverted repeat gene constructs into susceptible Indian potato cultivars.														DP, BPS & MNB
04	Validation of molecular markers Acquisition of molecular markers tightly linked to R-genes and synthesis of PCR primers														SKK
	Validation of PCR markers in late blight differentials														SKK
	Screening of parental lines and late blight resistant commercial cultivars, selection of parents and hybridization														SKK, BPS & MNB
	Molecular mapping of quantitative trait loci (QTL) for horizontal resistance in mapping population Validation of available SSR markers, DNA isolation and genotyping using SSR markers														SKK
	Conversion of SSR molecular marker data into linkage map using Join Map software														SKK
	Correlation of LB Phenotypic data with molecular map to identify the QTLs														SKK
05	Isolation of novel microorganism with activity against P. infestans														BPS & MNB
	Extraction and identification of microbial metabolites with activity against P. infestans														MAK, MNB & BPS
06	Collection of weather and disease data under different spray schedules using popular varieties														BPS & MNB
	Development of yield loss assessment models														BPS & MNB
	Development of Decision Support System														BPS & MNB
	Study effect of elevated temperature on host resistance, fungicide efficacy and aggression of P. infestans														BPS & MNB

BPS - B P Singh; MNB - M Narayana Bhat; DP - Debasis Patatnayak; SKC - S K Chakrabarti; SKK - S K Kaushik; MAK - M. A. Khan

3. ICAR-CPCRI, Kasaragod

S. No.	Activity	2008-09	2009-10				2010-11				2011-12				Work done by
		IV	I	II	III	IV	I	II	III	IV	I	II	III	IV
01	Collection of isolates of Phytophthora sp. causing diseases of coconut and cocoa from disease endemic areas, characterization, documentation and conservation. (Cultural and morphological characters, identification and pathogenic variability).														RC KD NR
	Studies on mating types and its geographical distribution														RC KD NR
	RAPD analysis and SSR analysis to find out the diversity. Molecular characterization of Phytopthora isolates based on ITS region of rDNA.														RC KD NR
02	Species specific/strain specific detection in host tissue and soil. Development of species – specific ITS primers for rapid detection of Phytophthora														RC KD NR
	Development of diagnostic kit for quick detection of the pathogen in soil and host tissue														RC KD NR
03	Effect of environmental factors on Phytophthora diseases, Source of the inoculum and effect of different cultural practices on Phytophthora population in soil.														RC KD NR
	Effect of environmental factors on Phytophthora diseases, Source of the inoculum and effect of different cultural practices on Phytophthora population in soil.														RC KD NR
	Developing disease forecasting systems with the help of prediction models.														RC KD NR
06	Seed / planting material and soil health management.														RC KD NR
	Integrated disease management involving cultural, biological and chemical control														RC KD NR
	Analysis of data and preparation of final report														RC KD NR

RC – R . Chandramohan; KD – K. Devakumar; N. Ravikumar

4.ICAR-CTCRI, Trivandrum

S. No.	Activity	2009-10				2010-11				2011-12				Work done by
		I	II	III	IV	I	II	III	IV	I	II	III	IV
01	Collection of isolates of the pathogen from different places and purification													RSM, VH & MLJ
	Morphological and cultural characterization of the pathogen isolates													RSM, VH & MLJ
	AFLP analysis of P. colocasiae isolates													RSM, VH & MLJ
	AFLP and SSR analysis of taro genotypes													RSM, VH & MLJ
02	Standardize the Real time PCR method for detection and quantification of pathogen propagules in planting material													RSM, VH & MLJ
	Field validation of molecular detection techniques like PCR for detection of pathogen in taro													RSM, VH & MLJ
03	Observation on field trials and recording the epidemiological factors													VH & MLJ
04	Identification of suitable promoter for elicitor gene isolated from P. colocasiae													RSM, VH & MLJ
05	Standardization of use of elicitor protein isolated from P. colocasiae for in vitro screening of taro genotypes against leaf blight													RSM, VH & MLJ
	Development of in vitro screening method for Colocasia using specific elicitor protein													RSM
	Mapping of taro genotypes to leaf blight													RSM, VH & MLJ
06	Preparation of bioformulation from the existing bio control agents against leaf blight													RSM, VH & MLJ
	Evaluation of suitable bioformulation technique in glass house													RSM, VH & MLJ
	Selection of effective bioformulation for control of tuber rot and leaf blight.													VH & MLJ
	Lay out field trial with bio-control formulation and IDM for management of leaf blight.													VH & MLJ
	Observation and recording the data on disease incidence in the IDM experiments													RSM, VH & MLJ
	On farm demonstration of IDM technology													RSM, VH & MLJ
	Observation of performance of IDM technology													RSM, VH & MLJ
	Indexing of planting material of taro for pathogen through PCR and ELISA													RSM
	Development of healthy planting material													RSM
	Multiplication of healthy planting materials													RSM, VH & MLJ
	Observation on performance of indexed planting material of taro at farmers’ field													RSM, VH & MLJ
	Data analysis and report writing													RSM, VH & MLJ

RSM - R. S. Misra; VH - Vinayaka Hegde; MLJ - M. L. Jeeva

5. ICAR-NRC Citrus, Nagpur

S. No.	Activity	2009-10				2010-11				2011-12				Work done by
		I	II	III	IV	I	II	III	IV	I	II	III	IV
	Procurement of equipments
	Recruitment of SRFs
01	Collection and conservation of Phytophthora spp. isolates													AKD
	Diversity analysis													AKD
02	Development of molecular diagnostic kit, quantification in host tissue and soil, large scale indexing													AKD
03	Host-pathogen and pathogen-pathogen interaction studies													AKD & VB
04	Host Resistance : Screening of citrus rootstocks to evaluate resistance against Phytophthora spp.													AKD & IPS
05	Management : Search for novel bioagents and testing promising bioagents against Phytophthora spp													AKD

AKD - A.K. Das; VB - V. Bamel; IPS - I. P. Singh

6.ICAR RC NEH, Umiam

S. No.	Activity	2008-09	2009-10				2010-11				2011-12				Work done by
		IV	I	II	III	IV	I	II	III	IV	I	II	III	IV
	Extensive literature search														RD/PB
01	Survey of Phytophthora in citrus prone in disease prone area of different agro-climatic zone.														RD/PB
	Isolation and characterization of Phytophthora spp. from plants as well as soil.														RD/PB
	Characterization Phytophthora isolates using PCR based molecular tool.														RD/PB
02	Isolation and characterization of antagonistic microbes														RD/PB
	Test the pathogenicity of collected isolates of Phytophthora spp.														RD/PB
	Test the bio-efficacy of bioagent against Phytophthora spp. under in vitro and protected conditions.														RD/PB
	Formulation studies and field evaluation of selected bioagents														RD/PB
03	Evaluation of available ITKs														RD/PB
	Conduct training to agriculture officers and extension worker on diagnosis and management of citrus diseases														RD/PB
	Submission of final report														RD/PB

RD - R. Dutta; PB - P. Baiswar

7.ICAR-YSPUHF,RC Kullu

S. No.	Activity	2008-09	2009-10				2010-11				2011-12				Work done by
		IV	I	II	III	IV	I	II	III	IV	I	II	III	IV
	Recruitment of staff (SRF)														IMS, DG, PG VS, BG, RK
	Procurement of Equipments														IMS, DG, PG VS, BG, RK
01	Survey for the collection of diseased samples														IMS, DG, PG VS, BG, RK
	Collection of soil samples from wilted and healthy plants														IMS, DG, PG VS, BG, RK
	Isolation of pathogen from wilted disease samples														IMS, DG, PG VS, BG, RK
	Estimation of total microbial population (Fungi, Bacteria and Actinomycetes) and nematode fauna form soil samples of healthy and wilted guava plants.														IMS, DG, PG VS, BG, RK
	Diversity analysis using phenotypic methods and other taxonomic methods														IMS, DG, PG VS, BG, RK
	Deposition of Fusarium isolates at NBAIM, Mau														IMS, DG, PG VS, BG, RK
02	Standardization of DNA isolation protocol of Fusarium isolates														IMS, DG, PG VS, BG, RK
	Screening of RAPD and STMS markers														IMS, DG, PG VS, BG, RK
	Identification of repeatable differentiating bands														IMS, DG, PG VS, BG, RK
	Elution of repeatable band														IMS, DG, PG VS, BG, RK
	Cloning of suitable vector (pGEMT / any T vector)														IMS, DG, PG VS, BG, RK
	Screening in M-13 phage vector														IMS, DG, PG VS, BG, RK
	Sequencing														IMS, DG, PG VS, BG, RK
	Designing of primers														IMS, DG, PG VS, BG, RK
	Validation of results														IMS, DG, PG VS, BG, RK
	Development of PCR based diagnostic kit														IMS, DG, PG VS, BG, RK
03	Weather data will be recoded weekly with the help of automatic weather station for its correlation with wilt incidence and development of prediction model														IMS, DG, PG VS, BG, RK
	Collection weather data														IMS, DG, PG VS, BG, RK
	Soil data will be recoded from healthy and wilted guava plants from hot spot (Major guava growing belt at Allahabad and Kanpur)														IMS, DG, PG VS, BG, RK
	Estimation of total microbial population Isolation of frequently occuring / domination fungi / bacteria and its screening against virulent Fusarium spp. isolates for its synergistic / antagonistic efficacy.														IMS, DG, PG VS, BG, RK
	Development of prediction models														IMS, DG, PG VS, BG, RK
03	Host Resistance Inoculation of Fusarium isolates to guava germ plasma using stem hole inoculation technique and observation on wilt incidence														IMS, DG, PG VS, BG, RK
	Identification of wilt resistant source														IMS, DG, PG VS, BG, RK
	Compilation of data, statistical analysis and compilation of report and submission of final report to New work Coordinator														IMS, DG, PG VS, BG, RK
04	Development of Integrated diseases management schedule (Field condition) • Evaluation of various co crops / intercrop (Ginger, Asperagus and Serpagandha) • Application of different Trichoderma harzianium, Trichoderma viride and Aspergiluus niger (Procured from PDBC, Bangalore) • Use of antagonistic crop (Marigold, Garlic and Turmeric) • Use of organic manure (Application of cow pat pit, Jeevamrit, Amritpani and Bijamrit)														IMS, DG, PG VS, BG, RK

IMS - I.M. Sharma; DG - D. Gupta; PG - P. Gupta; VS - Vinay Sagar; BG - Bhupesh Gupta; RK - Rajinder Kaur

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