Phytofura

Fusarium

1. ICAR-IARI, New Delhi

S. No.	Activity	2008-09	2009-10				2010-11				2011-12				Work done by
S. No.	Activity	IV	I	II	III	IV	I	II	III	IV	I	II	III	IV	Work done by
01	Diversity analysis of Fusarium oxysporum f. sp. ciceris (Min. 50 isolates) using RAPD, ISSR, SSR and ITS markers														SCD & PS
02	Development of race/species specific SCAR and rDNA based markers for detection of F. oxysporum f. sp. ciceris from host tissues/seeds and soils														SCD & PS
03	Validation of markers against different isolates of F. oxysporum f. sp. ciceris/ samples														SCD & PS
04	Screening of chickpea cultivars for standardization of differentials and for identification of broad based resistance against individual and multiple races														SCD & PS
05	Identification and functional analysis of genes potentially involved in the pathogenicity of F. oxysporum f. sp. ciceris														SCD & PS
06	Characterization of secondary metabolites produced by Trichoderma species														SCD & PS
	In vitro and in vivo evaluation of PGPRs for wilt management														SCD & PS
	Interaction of potential Trichoderma species with PGPRs (Rhizobium and Pseudomonas etc.) and fungicides and their effect on population dynamics and host plant														SCD & PS
	Development and evaluation of integrated wilt management modules based on consortium of effective bioagents in field														SCD & PS
07	Human resource development														SCD & PS

SCD – S C Dubey, PS - Parimal Sinha

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2. ICAR-IIPR, Kanpur

S. No.	Activity	2008-09	2009-10				2010-11				2011-12				Work done by
S. No.	Activity	IV	I	II	III	IV	I	II	III	IV	I	II	III	IV	Work done by
	Recruitment of contractual personnel and procurement of inputs														VD & RGC
01	Collection of new isolates of F. udum														VD & RGC
01	Diversity analysis of Fusarium udum using RAPD, ISSR, SSR, ITS and RGA markers.														VD & RGC
02	Cloning of pathogenesis related sequences and Development of SCAR markers for detection in host tissue and soil														VD & RGC
03	Impact of climate change														VD & RGC
05	Screening for resistance against individual and multiple races /variants and identification of broad based resistance														VD & RGC
06	Seed health management														VD & RGC
	PGPR and their role in disease management														VD & RGC
	Elicitors in imparting systemic resistance														VD & RGC
	Integrated disease management														VD & RGC
07	Human resource development														VD & RGC
07	Compilation of data, analysis and preparation of final report														VD & RGC

VD - Vishwa Dhar, RGC - R. G. Chaudhary

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3. ICAR-DOR, Hyderabad

S. No.	Activity	2008-09	2009-10				2010-11				2011-12				Work done by
S. No.	Activity	IV	I	II	III	IV	I	II	III	IV	I	II	III	IV	Work done by
01	Diversity analysis of Fusarium oxysporum f. sp. carthami using RAPD, ISSR, SSR, and ITS and EF1a seq. analysis.														RDP, KA, SCR & HK
01	Pathogenic variability studies.														RDP, KA, SCR & HK
02	Detection of Fusarium in early and mixed infections by RT -PCR.														RDP, KA, SCR & HK
02	SCAR markers for Fusarium oxysporum f. sp. carthami and its races.														RDP, KA, SCR & HK
05	Generation of mapping populations and selection of resistant genes by flanking markers														RDP, KA, SCR & HK
05	Identification of species specific molecular markers for targeted resistant genes in the wild species.														RDP, KA, SCR & HK
06	Field evaluation of potential bioagents (Trichoderma harzianum and T. viride) selected.														RDP, KA, SCR & HK
06	Role of Trichoderma species as elicitors in imparting systemic resistance														RDP, KA, SCR & HK

RDP - R. D. Prasad, KA - K. Anjani, SCR - S. Chander Rao, HK - H. Kumaraswamy

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4.ICAR-IIVR, Varanasi

S. No.	Activity	2009-10				2010-11				2011-12				Work done by
S. No.	Activity	I	II	III	IV	I	II	III	IV	I	II	III	IV	Work done by
01	Survey, collection, documentation and maintenance of isolates of tomato wilt pathogen in tomato from different agro-climatic zones													TKB, ML & RKS
	Characterization and diversity analysis of different isolates based on cultural and morphological molecular character of wilt pathogen													TKB, ML & RKS
	Characterization of different isolates based on pathogenic potential and selection of aggressive isolate													TKB, ML & RKS
	Characterization of pathogenic variants based on differential host reaction of tomato													TKB, ML & RKS
	Conservation of culture for repository for tomato wilt pathogen isolates													TKB, ML & RKS
02	Development of rapid PCR based molecular detection tools in host tissues and soil													TKB, ML & RKS
02	Development of markers for virulence													TKB, ML & RKS
03	Collection of weather parameters viz., temperature (minimum and maximum), humidity, rainfall during disease development in artificially inoculated plot													TKB, ML & RKS
	Analysis of the interaction of Host–pathogen – environment													TKB, ML & RKS
	Isolation of rhizosphere micro flora from the tomato rhizosphere and studying the interaction of the microflora with disease development in artificially inoculated conditions													TKB, ML & RKS
05	Identification of promising tomato genotypes with a good degree of resistance to most virulence and prevalence race of tomato wilt pathogen based on artificial and field screening and their use as one of the components in integrated disease management													TKB, ML & RKS
05	Extensive screening and evaluation of potential native biocontrol agents for integrated disease management													TKB, ML & RKS
06	In vitro evaluation of antagonist and new fungicides													TKB, ML & RKS
06	Integration of host resistance, chemical and biological for integrated disease management (IDM) in field													TKB, ML & RKS

TKB - T.K Bag, ML - M. Loganathan, RKS - R.K. Saritha

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5. ICAR-NRC, Banana, Trichy

S. No.	Activity	2009-10				2010-11				2011-12				Work done by
S. No.	Activity	I	II	III	IV	I	II	III	IV	I	II	III	IV	Work done by
01	Collection of Foc isolates from different banana growing regions in states like Bihar, West Bengal, Orissa, Gujarat, Maharastra Goa, Mizoram and Andaman and Nicobar islands (Not covered earlier)													RT & SB
	Development of more nit-M testers specific to Indian Foc isolates and grouping of all these Foc isolates by VCG analysis.													RT & SB
	Study on the pathogenic variation among the Foc isolates													RT & SB
	Phylogenetic grouping of all the identified VCG’s based on DNA sequences obtained from the nuclear translation elongation factor 1a (EF 1 a) and the mitochondrial small sub unit (mtSSU) ribosomal RNA genes													RT & SB
	Correlation of phylogenetic groups obtained from different molecular methods with pathogenicity to identify the clusters or clades specific to pathogenicity													RT & SB
02	Validation of SCAR and rDNA-ITS region targeted markers developed, in plants as well as in soil so as to put it for practical use													RT & SB
	Sequencing the Intergenic spacer region of the ribosomal DNA (rDNA) of a representative isolates of pathogenic and non-pathogenic Fusarium isolates													RT & SB
	Exploiting the genetic variation of IGS region to design primers specific for strains of Foc													RT & SB
	Development of a PCR-based assay and evaluation for its sensitivity and specificity under lab condition													RT & SB
	Detection and quantification of pathogenic Fusarium present in soil as well as in plants using Real –Time PCR assay.													RT & SB
03	Isolation, identification and evaluation of effective endophytic microbes such as non pathogenic F. oxysporum, fluorescent pseudomonas, and streptomyces against major clusters/ clades/ VCG,s/ races of Fusarium wilt pathogen under in vitro and in vivo conditions													RT & SB
	Development of consortium of biocontrol agents (by combining microbes having different mode of actions) and evaluation for their impact on disease suppression, and plant growth promotion													RT & SB
	Studying the mechanism of disease suppression by biocontrol agents including the quantification of transcript profiles/levels of defense associated genes coding for 1-3, ß-D- glucanases, Phenylalanine ammonia lyase (PAL), Chalcone synthases etc, in roots, corm and stem of the plants by applying real time QRT-PCR technology.													RT & SB
	Studying the bio-priming against Fusarium wilt disease by applying effective bio-agents in TC plants													RT & SB
	Evaluation of application of effective bio-agents along with/without fungicides for their disease suppression													RT & SB
04	Mass multiplication of resistant cultivars and susceptible cultivars													RT & SB
	Isolation of total RNA at different time intervals from Foc inoculated and un-inoculated plants													RT & SB
	cDNA -AFLP analysis of transcripts obtained from Foc inoculated and un-inoculated plants													RT & SB
	Cloning and sequencing of differentially expressed genes													RT & SB
	Characterization of gene sequence by bioinformatics tools													RT & SB
	Isolation of total RNA at different time intervals from inoculated and un-inoculated plants for the confirmation													RT & SB
	Real time PCR analysis for studying the expression level													RT & SB
05	Designing degenerate primers and identification of suitable primers for banana													RT & SB
	Cloning and sequencing of amplified RGAs													RT & SB
	Sequence alignment and phylogentic analysis													RT & SB
	Designing specific primers for banana RGA													RT & SB
	Identification of functional RGAs and pulling out full length cDNAs													RT & SB
	Validation of functional RGAs in resistant as well as susceptible cultivars													RT & SB

RT - R.Thangavelu, SB - S. Backiyarani

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6.ICAR-NBAIM. Mau

S. No.	Activity	2008-09	2009-10				2010-11				2011-12				Work done by
S. No.	Activity	IV	I	II	III	IV	I	II	III	IV	I	II	III	IV	Work done by
01	Conservation of isolates of different species of Fusarium Short term by slants, at 4 C Mineral oil lay on Long term by at -20 by glycerol stock and lyophilization														SK & AS
02	Genetic diversity of strains / isolates / pathotypes of Fusarium by RFLP .														SK & AS
03	DNA isolation ITS and IGS Amplification, RFLP														SK & AS
04	TEFAmplification, RFLP														SK & AS
05	Beta-tubulin Amplification, RFLP														SK & AS
06	Histone-3 Amplification, RFLP														SK & AS
06	Sequence alignment and analysis by comparative genomics sequencing of the above suitable targeted genes Editing of these sequences by using various bioinformatics tools Identification of the signature sequences Design of species specific primers and probe to the identified signature sequences														SK & AS
07	RAPD analysis for genotypic diversity by using • ERIC, REP, BOX and OPB primers														SK & AS
08	Development of database • Digitization for Indian isolates of Fusarium • Development of species specific barcodes														SK & AS

AS - Alok Srivastava, SK - Sudheer Kumar

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