Ralstonia
1. ICAR-IISR, Kozhikode
| S. No. | Activity | 2008-09 | 2009-10 | 2010-11 | 2011-12 | Work done by | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IV | I | II | III | IV | I | II | III | IV | I | II | III | IV | |||
| Recruitment of Staff |  | | | MA AK | |||||||||||
| Purchase of Equipment | | | | MA AK | |||||||||||
| 01 | Diversity analysis of Ralstonia solanacearum using molecular methods such as AFLP/Rep-PCR/16S rDNA sequencing and phenotypic methods such as Substrate utilization screening and biochemical methods | | | | | AK RSB | |||||||||
| 02 | Identification of race and behaviour specific sequence in Ralstonia solanacearum using molecular characterization/bioinformatics tools and development of diagnostic tools | | | | | AK RSB | |||||||||
| Validation of diagnostic tools in field samples such as soil and rhizomes | | | | | | | | AK RSB | |||||||
| 03 | Biochemical analysis of Indian mango ginger and deciphering of mechanism of bacterial wilt resistance | | | | | | | | | AK RSB | |||||
| Biochemical analysis of early events in the wilt induction and studies on the host response to the wilt pathogen Functional genomic approaches for studying the early events in the wilt induction | | | | | | | | | AK RSB | ||||||
| Studies on microbial interaction in vascular elements and understanding of the synergistic or antagonistic behavior of xylem borne microorganisms | | | | | | AK RSB | |||||||||
| Studies on the interaction of Nematodes/ Pythium/ Fusarium with Ralstonia especially in ginger | | | | | AK RSB | ||||||||||
| Genomic approaches for studying the fate of bacterium in soil or plant debris after the disease cycle | | | | | | | | AK RSB | |||||||
| 04 | Search for resistance in indigenous germplasm of major crops such as ginger and development of agronomically superior bacterial wilt resistance cultivars | | | | | | | | | AK RSB | |||||
| 05 | Isolation of microbial candidates for use against wilt pathogens | | | | | AK RSB | |||||||||
| Development of biological control methods for bacterial wilt and its validation in field situation and exploitation of BCA derived molecules for deployment against wilt disease. BCA derived elicitors are already reported for induction of Induced Systemic Resistance (ISR). | | | | | | | | | AK RSB | ||||||
| Development of Seed/Rhizome Health Management system | | | | | | | | | | | | | AK RSB | ||
| Development of soil treatment based on biofumigation using cruciferae family members | | | | | | | | | | | | AK RSB | |||
| Development of integrated disease management modules for wilt management | | | | | | | | | | | | AK RSB | |||
| Development of seed plot technique for ginger especially in endemic area of bacterial wilt | AK RSB | ||||||||||||||
MA – M. Anandaraj; AK – A. Kumar; RSB – R. Suseela Bhai
2. ICAR RC NEH, Umiam
| S. No. | Activity | 2008-09 | 2009-10 | 2010-11 | 2011-12 | Work done by | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IV | I | II | III | IV | I | II | III | IV | I | II | III | IV | |||
| Extensive literature search | RD/PB | ||||||||||||||
| 01 | Survey of bacterial wilt of tomato in diseases prone area of different agro-climatic zone. | | | | | | RD/PB | ||||||||
| Isolation and characterization of R. solanacearum infected plants as well as soil. | | | | | RD/PB | ||||||||||
| Characterization R. solanacearum isolates using PCR based molecular tool. | | | | | | | RD/PB | ||||||||
| Test the pathogenicity of collected isolates of R. solanacearum. | | | | | RD/PB | ||||||||||
| 02 | Isolation and characterization of antagonistic microbes especially Pseudomonas fluorescens and Bacillus spp. | | | | | RD/PB | |||||||||
| Test the bio-efficacy of bioagent against R. solanacearum under in vitro and protected conditions. | | | | | | | | RD/PB | |||||||
| Formulation studies and field evaluation of selected bioagents | | | | | | | RD/PB | ||||||||
| Evaluation of available ITKs | | | | | | | RD/PB | ||||||||
| 03 | Conduct training to agriculture officers and extension worker on diagnosis and management of bacterial wilt diseases | | | | | | RD/PB | ||||||||
| 04 | Submission of final report | | | RD/PB | |||||||||||
RD - R. Dutta; PB - P. Baiswar
3. ICAR-IARI, New Delhi
| S. No. | Activity | 2008-09 | 2009-10 | 2010-11 | 2011-12 | Work done by | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IV | I | II | III | IV | I | II | III | IV | I | II | III | IV | |||
| 01 | Collection of Ralstonia solanacearum from diseases prone area of different agro-climatic zone. | | | | | | DS/ KKM | ||||||||
| Diversity analysis of R. solanacearum using molecular methods such as AFLP, Rep- PCR/ 16s r DNA sequencing and phenotypic and biochemical methods. | | | | | | | | | DS/ KKM | ||||||
| Studies on microbial interaction in vascular elements and understanding of the synergistic or antagonistic behaviour of xylem borne microorganism | | | | | | KKM | |||||||||
| Test the pathogenicity of R. solanacearum isolates collected from different zones. | | | | | | | | | DS/ KKM | ||||||
| 02 | Identification of race/ biovar specific sequence in R. solanacearum by using molecular techniques/ bioinformatics tools and development of diagnostic tool. | | | | DS/ KKM | ||||||||||
| Validation and standardization of molecular diagnostic tool. | | | | | | | DS | ||||||||
| 03 | Studies on interaction of nematodes/ Fusarium with Ralstonia | | | | | | | DS | |||||||
| 04 | Analysis of virulence genes using bioinformatics | | | | | | | | DS | ||||||
| Genomic approaches for studying the fate of bacterium in soil or plant debris after the disease cycle. | | | | | KKM | ||||||||||
| 05 | Development of soil treatment based on biofumigation using cruciferae family members. | | | | | | | | DS | ||||||
| Isolation of microbial candidates for use against wilt pathogens. | | | | | | | DS/ KKM | ||||||||
| Development of biological control methods for bacterial wilt and standardize the inoculum potential and time of application of bioagent and its validation in field situation. | | | | | | | | DS | |||||||
| Development of integrated disease management modules for wilt management | | | | | | DS/ KKM | |||||||||
| Prepare database, documentation and report writing of the project. | | | | | | | | | | DS/ KKM | |||||
DS - Dinesh Singh; KKM
4.ICAR-IIHR, Bangalore
| S. No. | Activity | 2008-09 | 2009-10 | 2010-11 | 2011-12 | Work done by | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IV | I | II | III | IV | I | II | III | IV | I | II | III | IV | |||
| 01 | Survey for collection of various isolates of RS from the major tomato and brinjal growing areas in India | | | | | | CG, ATS, THS & KMR | ||||||||
| Biological characterization of RS isolates by host differentials under glass house condition | | | | | | CG, ATS, THS & KMR | |||||||||
| Biochemical characterization of strains of RS | | | | | | CG, ATS, THS & KMR | |||||||||
| Conservation of RS isolates for long term preservation | | | | | | CG, ATS, THS & KMR | |||||||||
| Molecular characterization of strains of RS isolates by Rep-PCR, ERIC-PCR, rDNA -PCR and PCR-RFLP analysis | | | | | | | | CG, ATS, THS & KMR | |||||||
| 02 | PCR based diagnostics for species, biovar, race and phylotype identification of RS isolates | | | | | | | | CG, ATS, THS & KMR | ||||||
| PCR based diagnosis of Ralstonia population in soil/host tissue | | | | | CG, ATS, THS & KMR | ||||||||||
| NASH based diagnosis for large scale detection of RS | | | | | | | | | CG, ATS, THS & KMR | ||||||
| 03 | Studies on host-pathogen-environment interaction in bacterial wilt disease epidemic | | | | | CG, ATS, THS & KMR | |||||||||
| Studies on interaction of Nematodes with RS | | | | CG, ATS, THS & KMR | |||||||||||
| 04 | Genetic characterization of hsp and endogluconase genes from severe and mild isolates of RS | | | | | | | | | CG, ATS, THS & KMR | |||||
| 05 | Screening of germplasm lines of tomato and brinjal for resistance to bacterial wilt | | | | | CG, ATS, THS & KMR | |||||||||
| 06 | Isolation and characterization of bio agents of RS | | | | | | | | | CG, ATS, THS & KMR | |||||
| Evaluation (lab. glasshouse & field) of chemicals, botanicals and bio agents against BW in tomato and brinjal | | | | | | | | | CG, ATS, THS & KMR | ||||||
| Development of IDM strategies for the management of BW in tomato and brinjal | | | | | | | | | CG, ATS, THS & KMR | ||||||
| Statistical analysis and compilation of reports | | | CG, ATS, THS & KMR | ||||||||||||
CG - C. Gopalakrishnan, ATS - A.T. Sadashiva, THS - T.H. Singh, KMR - K. Madhavi Reddy
5. ICAR-RC-Goa
| S. No. | Activity | 2008-09 | 2009-10 | 2010-11 | 2011-12 | Work done by | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IV | I | II | III | IV | I | II | III | IV | I | II | III | IV | |||
| 01 | Collection of R. solanacearum isolates infecting eggplant and chilli from wilt endemic locations of India | | | | | RR & MT | |||||||||
| Phenotypic characterization (pathogenicity, virulence, host range studies and biovar determination) of R. solanacearum isolates | | | | | RR & MT | ||||||||||
| Analysis of genetic diversity of R. solanacearum using molecular methods such as Rep-PCR, 16S rDNA sequencing and PCR-RFLP | | | | | RR & MT | ||||||||||
| 02 | Identification of race and biovar specific sequence in R. solanacearum using molecular characterization and bioinformatics tools and development of detection technique | | | | | RR & MT | |||||||||
| Study of the virulence factors using the sequence data and bioinformatics | | | | | | | RR & MT | ||||||||
| 03 | Study of native bacterial and R. solanacearum population dynamics in the xylem vascular tissue and their interaction | | | | | | | | | RR & MT | |||||
| 04 | Screening of eggplant germplasm comprising of cultivated types, exotic collections and wild relatives against R. solanacearum. | | | | | RR & MT | |||||||||
| Development of suitable mapping population (F1, F2, F3) for resistant gene tagging in eggplant. Phenotypic screening of F3 population for disease reaction | | | | | | | | RR & MT | |||||||
| Genotypic screening and establishment of marker-phenotype association using statistical analysis | | | | | RR & MT | ||||||||||
| 05 | Isolation of microbial candidates for use against R. solanacearum | | | | | RR & MT | |||||||||
| Development of biological control methods and their validation | | | | | | | | | RR & MT | ||||||
| Exploration of biocontrol agent derived elicitors and induced systemic resistance in enhancing host defense | | | | | | | | | RR & MT | ||||||
| Development of integrated bacterial wilt management modules | | | | | | | | | RR & MT | ||||||
RR - R. Ramesh; MT - M. Thangam