Protocol

Centre : Indian Institute of Oilseeds Research, Hyderabad
Name of the Protocol
Identification of SSR markers linked to Fusarium wilt resistance
Nature : Novel
Crop / Pathogen : Safflower/Fusarium oxysporum f. sp. carthami

Inventors

1. Dr K Anjani
2. Dr R D Prasad

Objectives

1. Introgression of Fusarium wilt resistance from wild species into cultivated safflower and marker-assisted selection for wilt resistance

Procedure / Summary

Fusarium wilt susceptible safflower (C. tinctorius L.), Nira was crossed to wilt resistant wild species, C. oxyacantha, C. palaestinus, C. lanatus, C. creticus, C. turkestanicus and C. galaucus. Screened F1-F7 generations against Fusarium wilt in wilt sick plot/pots. The plants with any intensity of wilt symptom were designated as susceptible and the one without wilt was designated as resistant. Single dominant gene control of wilt resistance was found. Of the 320 SSR markers used, 45 confirmed true hybriditity of interspecific hybrids. Bulked-segregant analysis employed in F2 mapping populations identified 34 species-specific SSRs linked to Fusarium wilt resistance. Using these markers, marker-assisted selection for wilt resistance was practiced successfully in F2-F7 generations of all interspecific crosses. Wilt resistant individuals in each generation could be selected at seedling stage using markers prior to commencement of wilt in the field. Total 116 wilt resistant interspecific inbred lines were derived through marker-assisted selection (MAS).

Applications

1. The SSR markers linked to wilt resistance are useful for selection of Fusarium wilt resistant individuals at seedling stage itself, and for pyramiding of wilt resistant genes from six wilt resistant wild species into cultivated species background to develop a broad and durable wilt resistant cultivar.

Publications

1. Anjani, K., Pallavi, M., Pradsa, R.D., Dinesh Kumar, V and Madanmohan, B. 2010. Relevance of marker-assisted breeding for Fusarium wilt resistance in safflower (Carthamus tinctorius L.). National Symposium on “Genomics and Crop Improvement Relevance and Reservations”, February 25-27,2010, Hyderabad.
2. K. Anjani, P. Bhavna, Debadutta Mishra, R.D. Prasad.2016. Exploitation of wild species, Carthamus oxyacantha Bieb. for disease resistance and yield improvement in safflower (C. tinctorius L.). Oral presentation during National Seminar on “Breeding of field crops for biotic and abiotic stress in relation to climate change” from 28-29 March 2016 at College of Agriculture, VNMKV, Parbhani. Souvenir and Book of Abstracts, pp-198.