Centre : Indian Institute of Oilseeds Research, Hyderabad
Name of the Protocol
Screening technique against Phytophthora nicotianae
Nature : Novel
Crop / Pathogen : castor, safflower/Phytophthora
Developed agar culture disc inoculation technique to screen and select resistance sources to Phytophthora leaf blight disease in castor and safflower. In this method, healthy mature castor/safflower leaves on 20 days-old plants can be selected and the reverse side of the leaves should be inoculated with Phytophthora agar discs (5mm) taken from actively growing edges of the culture. Agar bit is cover with small wet cotton and sealed with cellophane. Phytophthora used in this study has to be grown on carrot agar at 18 0C in the dark for 5 days. Leaf blight symptoms appears within 24-48h after inoculation and the leaves can be examined for lesion size and disease severity. Observations should be taken up to five days after inoculation.