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P h y t o p h t h o r a ....D i s e a s e s.... i n .....H o r t i c u l t u r a l ....C r o p s
 
   
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Protocols
  
   Protocols (Phytophthora Related)
  DNA isolation from Black Pepper (2% CTAB, modified Doyle and Doyle protocol)
  Genomic DNA isolation of Phytophthora(Cooke and Duncan (1997) protocol)
  Detection and isolation of phytophthora from soil(ANANDARAJ & SARMA, 1990)
  Protocol for isolation of phytophthora (Erwin & Ribeiro, 1996)
  Protocol for determination of mating type of phytophthora (Erwin & Ribeiro, 1996)
  Protocol for isolation of endophytic fungi from black pepper((Arnold et al 2001)
    Protocols (Ralstonia Related)
  Detection Method for Ralstonia Solancearum in Soil
  Culturing Methods-Ralstonia Solancearum
   Protocols (Nematodes Related)
  Nematode Extraction
  Culturing of Nematodes
DETECTION AND ISOLATION OF PHYTOPHTHORA FROM SOIL (ANANDARAJ & SARMA, 1990).
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  • Sieve the test soil to < 2 mm fractions.
  • Place 25g of the sieved soil in a petri dish and add 40-50 ml of glass distilled water to make a soil-water suspension, so that 2-3 mm of free water remains above the soil when the particles get settled.
  • Float twenty leaflets of Albizia falcataria with the adaxial surface in contact with water.
  • Incubate the plates at 26o (± 2o C) for 72 h.
  • Surface sterilize the infected baits with 0.1% mercuric chloride for 1 min.
  • Rinse with three changes of sterile water and plate it on PVPH selective medium (Tsao & Guy, 1977).
  • This method can also be done in beakers instead of petri dishes.
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