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P h y t o p h t h o r a ....D i s e a s e s.... i n .....H o r t i c u l t u r a l ....C r o p s
 
   
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Protocols
  
   Protocols (Phytophthora Related)
  DNA isolation from Black Pepper (2% CTAB, modified Doyle and Doyle protocol)
  Genomic DNA isolation of Phytophthora(Cooke and Duncan (1997) protocol)
  Detection and isolation of phytophthora from soil(ANANDARAJ & SARMA, 1990)
  Protocol for isolation of phytophthora (Erwin & Ribeiro, 1996)
  Protocol for determination of mating type of phytophthora (Erwin & Ribeiro, 1996)
  Protocol for isolation of endophytic fungi from black pepper((Arnold et al 2001)
    Protocols (Ralstonia Related)
  Detection Method for Ralstonia Solancearum in Soil
  Culturing Methods-Ralstonia Solancearum
   Protocols (Nematodes Related)
  Nematode Extraction
  Culturing of Nematodes
ISOLATION OF ENDOPHYTIC FUNGI FROM BLACK PEPPER(Arnold et al 2001).         Download

  • Pepper tissues were washed in running tap water and moved to the laminar flow hood where sections were cut with sterile scalel.
  • These sections were surface -sterilized by dipping in 0.525% sodium hypochlorite for 2 minutes.
  • Then sterilized using 70% ethanol for2 minutes and rinse the tissues in sterile distilled water.
  • Dry the tissues on sterile filter papper.
  • The edges of the sampled tissues were cut off and discarded.
  • Subsamples of remaining tissue measuring approximately 2*3mm were placed individually in petri dishes containing yeast- malt agar with 0.1%stock antibiotic solution.
  • Incubated in a chamber for 21 days at 12h light/dark cycles.
  • The plates were monitored regularly for the growth of endophytic fungi.
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