Cut out mycelial discs of 3mm size from the margins of 72hr old cultures of unknown Phytophthora isolate and that of the known isolate (A1 or A2) using a sterile cork borer.
Plate it on 1.5% Carrot agar medium [amended with ß- sitosterol (30mg/l)] or Ribeiro's synthetic medium (Ribeiro et al., 1993) in a sterile 9 cm Petri dish at a distance of 30mm apart.
Incubate the plates in dark at 21-240C, for 21 days.
Check the production of oospores by viewing under a microscope at every 7 days interval.
The presence of oospores shows that the isolates are of opposite mating type.
Under the authority of IISR, developed by
the Bioinformatics Centre
Protocols (Phytophthora
Related) 