Apple stem pitting virus

Family   : Betaflexiviridae
Genus : Foveavirus
Acronym : ASPV

Introduction

Apple stem pitting virus (ASPV) is associated with Top working of apple disease which is a serious constraint to the production of apple. The disease is characterized by curling, puckering, and necrosis on leaves. The other two viruses associated with top working disease are Apple stem grooving virus (ASGV) and Apple chlorotic leaf spot virus (ACLSV)

Major Diseases

Stem pitting disease

Geographical Distribution

Himachal Pradesh, Jammu and Kashmir

Particle Morphology

Flexuous particles

Transmission Method

Unknown

Detection

ELISA, RT-PCR

Host Range and Symptomatology

AppleCurling, puckering, and necrosis on leaves
Pear (Pyrus communis)Deformed fruits, along with occasional chlorotic spots on leaves

References

1 Apple stem pitting virus. [Distribution map].. 2012, Distribution Maps of Plant Diseases, No.April, pp Map 1120 (Edition 1).
2 Ajay Brakta; Anil Handa; Thakur, P. D.; Manica Tomar; Pardeep Kumar, Malus pumila 'Spy 227' and Apple stem pitting virus: graft incompatibility and epinasty.. 2015, VirusDisease, 26, 1/2, pp 92-96.
3 Brakta, A.; Sharma, U.; Thakur, P. D.; Handa, A., First report of pear stony pit disease from India.. 2013, New Disease Reports, 27, 1/2, pp 11.
4 Brakta, A.; Thakur, P. D.; Handa, A., First report of apple top working disease caused by viruses (Apple stem grooving virus, Apple chlorotic leaf spot virus, and Apple stem pitting virus) in apple in India.. 2013, Plant Disease, 97, 7, pp 1001-1002.
5 Dhir, S.; Ram, R.; Hallan, V.; Zaidi, A. A., Molecular characterization of an Indian variant of Apple stem pitting virus: evidence of recombination.. 2011, Journal of Plant Pathology, 93, 2, pp 471-478.
6 Dhir, S.; Tomar, M.; Thakur, P. D.; Ram, R.; Hallan, V.; Zaidi, A. A., Molecular evidence for Apple stem pitting virus infection in India.. 2010, Plant Pathology, 59, 2, pp 393.
7 Ferretti, L.; Hallan, V.; Rana, T.; Ram, R.; Dhir, S.; Negi, A.; Lakshmi, V.; Thockchom, T.; Zaidi, A. A.; Barba, M., Nucleotide analysis of pome fruit virus isolates detected in apple and pear samples from Italy and India.. 2010, Julius-Kühn-Archiv, No.427, pp 230.
8 Surender Kumar; Singh, R. M.; Raja Ram; Badyal, J.; Vipin Hallan; Zaidi, A. A.; Anupam Varma, Determination of major viral and sub viral pathogens incidence in apple orchards in Himachal Pradesh.. 2012, Indian Journal of Virology, 23, 1, pp 75-79.
9 Tanuja Rana; Anuradha Negi; Sunny Dhir; Tombisana Thockchom; Vanita Chandel; Yashika Walia; Singh, R. M.; Raja Ram; Vipin Hallan; Zaidi, A. A., Molecular diagnosis of apple virus and viroid pathogens from India.. 2011, Archives of Phytopathology and Plant Protection, 44, 6, pp 505-512.

Sequences

Complete Sequence
 LM999967.1 Apple stem pitting virus, strain N, complete genome.
 FR694186.1 Apple stem pitting virus complete genome, isolate Palampur.
Partial Sequence
 FJ970956.1 Apple stem pitting virus isolate A3ASP1 coat protein gene, partial cds.
 KR815876.1 Apple stem pitting virus isolate Uttrakhand coat protein gene, complete cds.
 KR815875.1 Apple stem pitting virus isolate Solan coat protein gene, complete cds.
 KM873721.1 Apple stem pitting virus coat protein gene, complete cds.
 KM873720.1 Apple stem pitting virus coat protein gene, complete cds.
 KJ617060.1 Apple stem pitting virus isolate Red Delicious coat protein gene, partial cds.
 KJ617059.1 Apple stem pitting virus isolate Scarlet Gala coat protein gene, partial cds.
 KJ617058.1 Apple stem pitting virus isolate Royal Delicious coat protein gene, partial cds.
 KC154859.1 Apple stem pitting virus isolate ASPV-WRD nonfunctional coat protein gene, partial sequence.
 KC154860.1 Apple stem pitting virus isolate ASPV-Bartlett nonfunctional coat protein gene, partial sequence.
 HE574817.1 Apple stem pitting virus partial RdRp gene for polyprotein, RNA-dependent RNA polymerase region, isolate PAS, genomic RNA.
 FR750244.1 Apple stem pitting virus partial CP gene for coat protein, isolate Hatkoti, genomic RNA.
 FR694922.1 Apple stem pitting virus CP gene for coat protein, genomic RNA, isolate Rhoru.
 FN433599.1 Apple stem pitting virus TGB ORF genes and CP gene, genomic RNA, isolate Palampur.
 FM863704.1 Apple stem pitting virus partial cp gene for coat protein, clone SD3, genomic RNA.
 FN430678.1 Apple stem pitting virus partial CP gene for coat protein, genomic RNA, isolated from pear leaf in Palampur.
 FM212638.1 Apple stem pitting virus partial cp gene for coat protein, clone SD2, genomic RNA.
 FM212637.1 Apple stem pitting virus partial cp gene for coat protein, clone SD1, genomic RNA.
 FN395316.1 Apple stem pitting virus partial mRNA for RNA Polymerase (RdRp gene), isolate Palampur.
 FM863705.1 Apple stem pitting virus partial cp gene for coat protein, clone SD4, genomic RNA.