Okra enation leaf curl virus

Family   : Geminiviridae
Genus : Begomovirus
Acronym : OELCuV

Introduction

An unusual symptom in okra originating from Haryana state consisting of leaf curl associated with enations. These were distinct from the leaf curl and/or vein yellowing symptoms usually shown by okra. The causal virus of this new disease was identified as a distinct species namely Okra enation leaf curl virus (OELCuV). Analysis of the transmission characteristics of a wild-type virus isolate by Bemisia tabaci showed the minimum acquisition access period to be 1 h and the minimum inoculation access period to be 30 min, with female insects transmitting with a greater efficiency than male insects. Under controlled conditions the host range of the virus was shown to be very narrow, limited to two species in the family Malvaceae, okra (Abelmoschus esculentus) and hollyhock (Althaea rosea), and seven in the family Solanaceae. Together with okra yellow vein disease and okra leaf curl disease, okra enation leaf curl disease (OELCuD) causes severe losses in cultivated okra (Abelmoschus esculentus (L.) Moench) in India. The disease initially causes small pin-head enations on the under surface of leaves. This is followed by a warty and rough texture of leaves, with later leaves curling upwards. Affected plants show a twisting of the stem and lateral branches with leaves becoming thick and leathery. The curling and enations are more prevalent on leaves that develop soon after infection than in later leaves and plants are severely stunted with fruit being small, deformed and unfit for marketing.

Major Diseases

Enation leaf curl disease

Geographical Distribution

Haryana

Particle Morphology

Geminate particles

Transmission Method

By whitefly, Bemisia tabaci

Detection

PCR

Host Range and Symptomatology

Okra (Bhendi)Leaf curl associated with enations.

References

1 Chakraborty, S. ; Pandey, P. K. ; Singh, B., Okra enation leaf curl disease - a threat to cultivation of okra (Abelmoschus esculentus (L.) Moench).. 1997, Vegetable Science, 24, 1, pp 52-54.
2 Singh, B. ; Mathurai Rai ; Kalloo, G. ; Satpathy, S. ; Pandey, K. K., Wild taxa of okra (Abelmoschus species): reservoir of genes for resistance to biotic stresses.. 2007, Acta Horticulturae, No.752, pp 323-328.
3 Singh, S. J., Etiology and epidemiology of whitefly-transmitted virus diseases of okra in India.. 1990, Plant Disease Research, 5, 1, pp 64-70.
4 Singh, S. J. ; Dutta, O. P., Enation leaf curl of okra - a new virus disease.. 1986, Indian Journal of Virology, 2, 1, pp 114-117.
5 Venkataravanappa, V. ; Reddy, C. N. L. ; Devaraju, A. ; Salil Jalali ; Reddy, M. K., Association of a recombinant Cotton leaf curl Bangalore virus with yellow vein and leaf curl disease of okra in India.. 2013, Indian Journal of Virology, 24, 2, pp 188-198.
6 Venkataravanappa, V. ; Reddy, C. N. L. ; Salil Jalali ; Briddon, R. W. ; Reddy, M. K., Molecular identification and biological characterisation of a begomovirus associated with okra enation leaf curl disease in India.. 2015, European Journal of Plant Pathology, 141, 2, pp 217-235.

Sequences

Complete Sequence
 KT390463.1 Okra enation leaf curl virus isolate WOK8 segment DNA A, complete sequence.
 KT390460.1 Okra enation leaf curl virus isolate WOK10 segment DNA A, complete sequence.
 KT390459.1 Okra enation leaf curl virus isolate WOK333 segment DNA A, complete sequence.
 KT390458.1 Okra enation leaf curl virus isolate WOK330 segment DNA A, complete sequence.
 KT390457.1 Okra enation leaf curl virus isolate WOK329 segment DNA A, complete sequence.
 KT390454.1 Okra enation leaf curl virus isolate WOK42 segment DNA A, complete sequence.
 KT390452.1 Okra enation leaf curl virus isolate WOK5 segment DNA A, complete sequence.
 KT390450.1 Okra enation leaf curl virus isolate WOK2 segment DNA A, complete sequence.
 KT390346.1 Okra enation leaf curl virus isolate OK292 segment DNA A, complete sequence.
 KT390345.1 Okra enation leaf curl virus isolate OK427 segment DNA A, complete sequence.
 KT390344.1 Okra enation leaf curl virus isolate OK440 segment DNA A, complete sequence.
 KT390343.1 Okra enation leaf curl virus isolate OK146 segment DNA A, complete sequence.
 KT390342.1 Okra enation leaf curl virus isolate OK406 segment DNA A, complete sequence.
 KT390341.1 Okra enation leaf curl virus isolate OK10 segment DNA A, complete sequence.
 KT390340.1 Okra enation leaf curl virus isolate OK439-BR segment DNA A, complete sequence.
 KT390339.1 Okra enation leaf curl virus isolate OK435-BR segment DNA A, complete sequence.
 KT390338.1 Okra enation leaf curl virus isolate OK434-BR segment DNA A, complete sequence.
 KT390337.1 Okra enation leaf curl virus isolate OK431-BR segment DNA A, complete sequence.
 KT390336.1 Okra enation leaf curl virus isolate OK423-BR segment DNA A, complete sequence.
 KT390335.1 Okra enation leaf curl virus isolate OK403-BR segment DNA A, complete sequence.
 KT390334.1 Okra enation leaf curl virus isolate OK401-BR segment DNA A, complete sequence.
 KT390333.1 Okra enation leaf curl virus isolate OK414-BR segment DNA A, complete sequence.
 KT390332.1 Okra enation leaf curl virus isolate OK408-BR segment DNA A, complete sequence.
 KT390331.1 Okra enation leaf curl virus isolate OK385B-MP segment DNA A, complete sequence.
 KT390330.1 Okra enation leaf curl virus isolate OK375-MP segment DNA A, complete sequence.
 KT390329.1 Okra enation leaf curl virus isolate OK363-MP segment DNA A, complete sequence.
 KT390328.1 Okra enation leaf curl virus isolate OK357-MP segment DNA A, complete sequence.
 KT390327.1 Okra enation leaf curl virus isolate OK352-MP segment DNA A, complete sequence.
 KT390326.1 Okra enation leaf curl virus isolate OK311-RAJ segment DNA A, complete sequence.
 KT390322.1 Okra enation leaf curl virus isolate OK305-RAJ segment DNA A, complete sequence.
 KT390320.1 Okra enation leaf curl virus isolate OK296-RAJ segment DNA A, complete sequence.
 KT390319.1 Okra enation leaf curl virus isolate OK300-RAJ segment DNA A, complete sequence.
 KT390318.1 Okra enation leaf curl virus isolate OK291-RAJ segment DNA A, complete sequence.
 KT390317.1 Okra enation leaf curl virus isolate OK143-PUNJ segment DNA A, complete sequence.
 KT390316.1 Okra enation leaf curl virus isolate OK139-PUNJ segment DNA A, complete sequence.
 KT390315.1 Okra enation leaf curl virus isolate OK137-PUNJ segment DNA A, complete sequence.
 KT390314.1 Okra enation leaf curl virus isolate OK115-PUNJ segment DNA A, complete sequence.
 KT390313.1 Okra enation leaf curl virus isolate OK114-PUNJ segment DNA A, complete sequence.
 KT390312.1 Okra enation leaf curl virus isolate OK113-PUNJ segment DNA A, complete sequence.
 KT390311.1 Okra enation leaf curl virus isolate OK111-PUNJ segment DNA A, complete sequence.
 KT390310.1 Okra enation leaf curl virus isolate OK92-HR segment DNA A, complete sequence.
 KT390309.1 Okra enation leaf curl virus isolate OK91-HR segment DNA A, complete sequence.
 KT390308.1 Okra enation leaf curl virus isolate OK90-HR segment DNA A, complete sequence.
 KT390307.1 Okra enation leaf curl virus isolate OK89-HR segment DNA A, complete sequence.
 KT390306.1 Okra enation leaf curl virus isolate OK88-HR segment DNA A, complete sequence.
 KT390305.1 Okra enation leaf curl virus isolate OK87-HR segment DNA A, complete sequence.
 KT390304.1 Okra enation leaf curl virus isolate OK86-HR segment DNA A, complete sequence.
 KT390303.1 Okra enation leaf curl virus isolate OK11-UP segment DNA A, complete sequence.
 KT390302.1 Okra enation leaf curl virus isolate OK9-UP segment DNA A, complete sequence.
 KT390301.1 Okra enation leaf curl virus isolate OK8-UP segment DNA A, complete sequence.
 KP208672.1 Okra enation leaf curl virus isolate India:Ludhiana:2014 segment DNA-A, complete sequence.
 KC019309.1 Okra enation leaf curl virus isolate India:Gandhinagar:2012 clone 1 segment DNA-A, complete sequence.
 KC019308.1 Okra enation leaf curl virus isolate India:Gandhinagar:2012 clone 3 segment DNA-A, complete sequence.
 NC_014894.1 Okra enation leaf curl virus [India:Munthal EL37:2006] DNA-A, complete genome.
 GU112002.1 Okra enation leaf curl virus [India:Munthal EL38:2006] segment DNA-A, complete sequence.
 GU112001.1 Okra enation leaf curl virus [India:Munthal EL39:2006] segment DNA-A, complete sequence.
 GU112000.1 Okra enation leaf curl virus [India:Munthal EL32:2006] segment DNA-A, complete sequence.
 GU111999.1 Okra enation leaf curl virus [India:Munthal EL41:2006] segment DNA-A, complete sequence.
 GU111998.1 Okra enation leaf curl virus [India:Munthal EL37:2006] segment DNA-A, complete sequence.
 GU111997.1 Okra enation leaf curl virus [India:Sonipat EL14:2006] segment DNA-A, complete sequence.
 GU111996.1 Okra enation leaf curl virus [India:Sonipat EL10:2006] segment DNA-A, complete sequence.
Partial Sequence
 KT935487.1 Okra enation leaf curl virus isolate Vijayawada coat protein (AV1) gene, complete cds.
 KT898976.1 Okra enation leaf curl virus isolate Vijayawada coat protein (AV1) gene, complete cds.
 KT898975.1 Okra enation leaf curl virus isolate Hyderabad coat protein (AV1) gene, complete cds.
 KT898974.1 Okra enation leaf curl virus isolate Varanasi coat protein (AV1) gene, complete cds.
 KR068481.1 Okra enation leaf curl virus isolate Theni MKU-2 alpha karyopherin and AC1 genes, partial cds.
 KC342220.1 Okra enation leaf curl virus isolate Surat AV2, coat protein, AC5, Ren, TrAP, Rep, and AC4 genes, complete cds.