LOCUS       EF693942                1798 bp    DNA     linear   INV 22-MAY-2008
DEFINITION  Radopholus similis GHF5 endo-1,4-beta-glucanase precursor (eng2)
            gene, complete cds.
ACCESSION   EF693942
VERSION     EF693942.1  GI:157411572
KEYWORDS    .
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Haegeman,A., Jacob,J., Vanholme,B., Kyndt,T. and Gheysen,G.
  TITLE     A family of GHF5 endo-1,4-beta-glucanases in the migratory
            plant-parasitic nematode Radopholus similis
  JOURNAL   Plant Pathol. 57, 581-590 (2008)
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Haegeman,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2007) Molecular Biotechnology, Ghent University,
            Coupure Links 653, Ghent 9000, Belgium
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Radopholus similis"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:46012"
     gene            <436..>1451
                     /gene="eng2"
                     /note="Rs-eng2"
     mRNA            join(<436..1381,1438..>1451)
                     /gene="eng2"
                     /product="GHF5 endo-1,4-beta-glucanase precursor"
     CDS             join(436..1381,1438..1451)
                     /gene="eng2"
                     /EC_number="3.2.1.4"
                     /note="Rs-ENG2; endoglucanase; cellulase (glycosyl
                     hydrolase family 5)"
                     /codon_start=1
                     /product="GHF5 endo-1,4-beta-glucanase precursor"
                     /protein_id="ABV54448.1"
                     /db_xref="GI:157411573"
                     /translation="MLSLFLLPLFFAFASAANPPYGQLSVSGTKLKGSSGQDVALHGM
                     SLFWSNFGEGAPFYNAATVKALKCKWNSNIVRAAMGVEGGYLNNPSQEQAKLEAVVQA
                     AIAEGIYVIIDWHDHNAQSHQSQAVSFFTAMSKKYGKNTNILYEIFNEPLQVDWNSVV
                     KPYAQAVIKAIRANDAKNVIIVGTPTWSQDVDVAANSPITGQKNIMYTLHYYAATHKQ
                     DLRNKLTTAVNKGLPVFVTEYGTVTADGNGYVDAAESKTWWNFLDGLKISYVNWAIDN
                     KDEKSAALTPGTSSSQVGDSSRWTESGKLVQAKLASQKNGVSC"
ORIGIN      
        1 atccaaaatc gggcgagatc acgataccgc tcaacactag cgaaaccacg ttcagtttgg
       61 gaaaggtctt tttggttttg gccttctttt tcgcgttcgg aatttggtgg ccaccaaccg
      121 tgtagtcaaa caaaggtatg cactatttag ttggttgcgt ttaagtgcgg caacgaatca
      181 cgatcaaaac tgaatggggt tcctagctgt gcaaataatc taaagtgcgt tctctgggtt
      241 tgactytcac tgacgcaaaa ttcaaaagcc aaaatggaga atccaagtaa agtgcaaatt
      301 tgcacacgtt tggagcggca acatgacatc caagtgcagt gcaactaagt gcctttaaaa
      361 gtggaccaaa tcccccaaac atcaccactc attcattaat tccccatccg cagaagacca
      421 ttctttcaaa caactatgct tagcctgttc cttttgccgt tgttcttcgc ttttgccagc
      481 gcagccaacc cgccctacgg acagctgtcc gtctccggca ccaagctgaa gggctcctcc
      541 ggccaggacg tggccctgca cggcatgtcc ctgttctgga gcaacttcgg cgagggcgcc
      601 cccttctaca acgcggccac cgtgaaggcc ctcaagtgca aatggaactc gaacatcgtg
      661 cgcgcggcca tgggcgtgga gggcggctac ctgaacaacc cgtcccagga gcaggccaag
      721 ctggaggccg tggtgcaggc ggccatcgcg gagggcatct acgtgatcat cgactggcac
      781 gaccacaacg cccagagcca ccagagccag gcggtctcct tcttcacggc catgtccaag
      841 aagtacggaa agaacacgaa catcctctac gagatcttca acgagcccct gcaagtggac
      901 tggaacagcg tggtgaagcc ctacgcacag gccgtcatca aggccatccg cgccaacgac
      961 gccaagaacg tgatcatcgt gggcactccg acctggtccc aggacgtgga cgtagccgcg
     1021 aacagtccga tcacgggcca aaagaacatc atgtacacgc tgcactacta cgccgccacg
     1081 cacaaacagg acctgcgcaa caagctgacc accgccgtca acaagggcct gcccgtgttc
     1141 gtcaccgagt acggcacagt gactgccgac ggaaatggct acgtggacgc ggccgagtcc
     1201 aagacctggt ggaacttcct ggacgggctc aagatctcct acgtgaactg ggccatcgac
     1261 aacaaggacg agaagtctgc cgccctgacg cccggcacca gctcctccca agtgggcgac
     1321 tccagccgct ggaccgagtc cggcaaactg gtgcaggcca aactggccag ccaaaagaac
     1381 ggtcagtccc gaatcgggtt aactggctat taatgaatgc ctgatttgac tcttcaggtg
     1441 tgagctgctg atgatggacg agcaaatgca aaaagtgaat gagagatgac agatgagggt
     1501 ccaaaatttg cctagattgg tgtcaaaatt tcatagtcgc tatgcgtgta aaacgcaatt
     1561 ccaaaatgtt gctgtttaat agattattct gtgatgccaa tgagtcagac acacattaaa
     1621 agagcaaaca ccgacctgtc tcgctccgac tcgcacactc gtactgctcc gataagttgt
     1681 tcattgtggc tggcgaaaga acaaggttga gacattttga tgtgcaaacg ttacttcctc
     1741 cttgctagaa gtgatcgaat gattccacat cattttcaaa gtcacaagtt gtttgttt
//