LOCUS       EF693941                2683 bp    DNA     linear   INV 22-MAY-2008
DEFINITION  Radopholus similis GHF5 endo-1,4-beta-glucanase precursor (eng1B)
            gene, complete cds.
ACCESSION   EF693941
VERSION     EF693941.1  GI:157411570
KEYWORDS    .
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Haegeman,A., Jacob,J., Vanholme,B., Kyndt,T. and Gheysen,G.
  TITLE     A family of GHF5 endo-1,4-beta-glucanases in the migratory
            plant-parasitic nematode Radopholus similis
  JOURNAL   Plant Pathol. 57, 581-590 (2008)
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Haegeman,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2007) Molecular Biotechnology, Ghent University,
            Coupure Links 653, Ghent 9000, Belgium
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Radopholus similis"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:46012"
     gene            <427..>2451
                     /gene="eng1B"
                     /note="Rs-eng1B"
     mRNA            join(<427..642,749..946,993..1287,1360..1544,1589..1658,
                     1765..2039,2287..>2451)
                     /gene="eng1B"
                     /product="GHF5 endo-1,4-beta-glucanase precursor"
     CDS             join(427..642,749..946,993..1287,1360..1544,1589..1658,
                     1765..2039,2287..2451)
                     /gene="eng1B"
                     /EC_number="3.2.1.4"
                     /note="Rs-ENG1B; endoglucanase; cellulase (glycosyl
                     hydrolase family 5)"
                     /codon_start=1
                     /product="GHF5 endo-1,4-beta-glucanase precursor"
                     /protein_id="ABV54447.1"
                     /db_xref="GI:157411571"
                     /translation="MCCASALLGLLCLATFFASATALTATAPPYGQLSVSGTKLLSSS
                     GTAVALHGNSLFWSQWYPDFWTASVVQQLKCNWNANIVRAAMGVDQGGYLTTASTQYA
                     LVVAVVEAAITHGIYVIVDWHVSATYQSDAVAFFTKVSAAYGSYPHVLYETYNEPTSV
                     SWTDVLVPYHKAVIAAIRANDADNIIICGTPTWSQDVEVASANPITGYSNIMYTFHYY
                     AATHGASYRSKVTTAVNNGLPVFVTEYGTCESSGSGTIDSSSSATWWTFLDGLGISYV
                     NWAIDNKDETCAALTTSGSASTVGSSSYWSTSGKLVNAQQVAQSNGVSCSSSATTTTT
                     TTKSGATTTTTKASATTTTTKASATTTTTKASSSSSSSVTASVSTTNSWSGGSQVAIT
                     FKNSGSSSVCTIKFTITLPSGTTISSIWNASVVSGTTYTTASYFSLAAGSSDSSIGMV
                     LTGSGTPTVSIVSTSGC"
ORIGIN      
        1 ctgcacaaat tttgggcatt cgagaattcg agagagtacg cattccatgg aattttcaaa
       61 ggcaaacaaa tggcatcgca gatccactcc attatcggtt catacgcgag ttcgttggcg
      121 caattgggtg ggcaaatcgc agccacatga tgccgaactt tggcgttaaa gatatggctt
      181 tttaattttt gcggctgaac aaatatagca aattgaggca ccacacagaa ttcgctaaat
      241 ggattggttg aatgagctga attaaagtac aaaacttggc ggaggtcagg gccaggccgc
      301 caccgttttg cggagccaaa catcaatcaa tcgttcgtgc ctttggtgag cacataaata
      361 ggcgacttca cttccacaaa atcacacaaa tccgctttca ccgctttcac ctctattcgc
      421 ccaaaaatgt gctgcgcctc tgcactgctc ggcttgctct gtttggcgac atttttcgcc
      481 tcagccactg cattgactgc caccgctccg ccatacggcc aactgtccgt gtccggcacc
      541 aagctgctga gctccagtgg cactgctgtg gcgttgcacg gcaactcgct gttctggtcg
      601 caatggtacc cagacttctg gaccgcatcc gtcgtgcaac aggtgagcgc tgagttctgt
      661 tttcgaaact ttgaagtcaa tctgcttctt tttcaaattt tggcattttg ggatctatcg
      721 acaaatatgg cattttgacg cctttcagct caaatgcaac tggaatgcga acattgtgcg
      781 tgccgctatg ggagtggacc agggcggcta tttgaccacc gcgagcacgc agtacgcgct
      841 cgtcgttgcc gtggttgagg cggccattac acacggtatc tacgtgatcg tggactggca
      901 cgtcagcgcc acttaccagt ccgacgcggt ggccttcttc accaaggtct gtgcttcggt
      961 gccatttttt ctgatgctta tccccacaat aggtgtccgc tgcgtacggc tcctacccgc
     1021 atgtgctgta cgagacctac aatgagccca cctctgtctc gtggacggat gttttggtgc
     1081 cataccacaa ggcggtgatt gccgccattc gcgccaacga cgcggacaac atcatcattt
     1141 gcggcacgcc cacatggtcc caggacgtgg aagtcgcatc cgcgaaccca atcaccggct
     1201 actccaacat catgtacaca ttccactact acgcagccac gcatggtgcc tcctaccgct
     1261 ccaaggttac caccgctgtg aacaacggta agtcgaaaaa ggaccggaac aatgaattgg
     1321 gggcttaatc gatcaactat tgattggcac tttgttcagg cctgcccgtg ttcgtgaccg
     1381 agtacggcac atgcgagtcg tcgggcagtg gcacaatcga ctcttcctcc tcggccactt
     1441 ggtggacctt cttggatgga ctgggaatct cttacgtgaa ctgggccatc gacaacaagg
     1501 acgagacttg cgctgcgctc accaccagcg gatccgcctc caccgttcga ttcatacgca
     1561 atttggtgcg atcaattttt gcgttcaggt cggcagctct tcctattggt ccacgtcggg
     1621 caaactggtc aacgcacaac aagtcgctca gagcaacggt aaaagcaatt tggttattgg
     1681 ctcaattcat gcgtaaatag cgtagatgaa aattacattc aaaatctggt tttccgatga
     1741 attgaaccaa actgtcatgt tcaggagtga gctgctcttc gtccgcgacc actaccacca
     1801 ccacgactaa atctggagcg accaccacta ccaccaaggc atcggccacc accactacca
     1861 ccaaggcctc ggccaccact accaccacaa aggcttcttc gtcctcgtcg agctcagtga
     1921 ccgcttcggt gtcgaccacc aactcgtgga gcggcggcag ccaagtggcc atcacgttca
     1981 agaactccgg ctcctccagt gtgtgcacca tcaagttcac gatcacactg ccgtccgggg
     2041 tgagtgcaaa tttgtcagga agatatgatt tccgtccaaa catgggcatc caaattaagt
     2101 agtgaatttg aactttgttc ggatcgaaat gtcttataag gctctccata tgttaaccaa
     2161 taaccactct ggacaaaacg atcacttttg atcactacca atggattctg atcaaaatcg
     2221 atcaaatgac tggaaccaaa aaattaaatt ttctacatta ttagaatttt tctcctcatt
     2281 ttgtagacca ccatctcgag catttggaac gccagtgtgg tcagcggcac cacctacaca
     2341 acggccagct acttcagtct ggcggccggc tcctccgaca gctccatcgg aatggtgctg
     2401 accggcagcg gcactcccac tgtctcaatt gtgtccacca gtggatgctg aatgtctggc
     2461 aggagatgaa gtacgagaga aagcgaactt ggaaaatttt acaaacgaat gaaagtgtcg
     2521 cctaaaaggg caatattgcg aaatgtcgag catataatcg tgtttttagt tgagtaattg
     2581 tctaattgtt agtaaattga tcatccaaag aaggacagtt ttggcgcaat ccggctcgag
     2641 ctagggagga ctaaatggga acgagcatca tatttgaatt gcg
//