LOCUS       EF208206                 706 bp    DNA     linear   INV 05-FEB-2007
DEFINITION  Radopholus similis isolate HN13 18S ribosomal RNA gene, partial
            sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene,
            and internal transcribed spacer 2, complete sequence; and 28S
            ribosomal RNA gene, partial sequence.
ACCESSION   EF208206
VERSION     EF208206.1  GI:124303179
KEYWORDS    .
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Peng,D., Geng,L., Zhang,D. and Li,Y.
  TITLE     Molecular diagnosis of quarantine nematode Radopholus similis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 706)
  AUTHORS   Peng,D., Geng,L., Zhang,D. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2007) Plant Nematology Laboratory, Institute of
            Plant Protection, Chinese Academy of Agricultural Sciences, West
            Yuanmingyuan Road No. 2, Beijing 100094, P.R. China
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Radopholus similis"
                     /mol_type="genomic DNA"
                     /isolate="HN13"
                     /db_xref="taxon:46012"
                     /PCR_primers="fwd_seq: cgtaacaaggtagctgtaggt, rev_seq:
                     tcctccgctaaatgatatg"
     rRNA            <1..42
                     /product="18S ribosomal RNA"
     misc_RNA        43..316
                     /product="internal transcribed spacer 1"
     rRNA            317..474
                     /product="5.8S ribosomal RNA"
     misc_RNA        475..668
                     /product="internal transcribed spacer 2"
     rRNA            669..>706
                     /product="28S ribosomal RNA"
ORIGIN      
        1 cgtaacaagg tagctgtagg tgaacctgct gcaggatcat tacttacaag tgaacctatc
       61 caatacgatt ccgtcctttg gtgggcagtg cccctcaggc attggcgaaa cccatcaaat
      121 gtgacgcgaa cggctgggtt ggcgtctgtg agtcgtggag cagttgtagt ccatgtccgt
      181 ggctgcgatg atgcgactcg gtagggctgt catcgccttt ggcagcttaa gacttgatga
      241 gcgcagacca agcgccgcca acaaccattt ttttccaata aactttttca aatgccattc
      301 aaggcaaaca agaattctag ccttatcggt ggatcactcg gctcgtaggt cgatgaagaa
      361 cgcagccagc tgcgatatct agtgtgaact gcagaacctt tgaacactaa acattcgaat
      421 gcacattgcg ccattggagt cacttcctct ggcacgcctg gttcagggtc gtttaccaaa
      481 aacgcaagac aaatgcgtac atgaatgcgt gataattaat attttcatgc aatggaaatt
      541 cagagtttgc taatttttta cttggacatg aatttcgtga gtatttgccg tgtggcgaaa
      601 gaaattcacg tatgttcatt gtgcaaattg gcagcttttc caaatcattc gacctgaact
      661 cagacgtgaa tacccgctga acttaagcat atcatttagc ggagga
//