LOCUS       EY195901                 477 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab16h05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAK83926.1|AF401090_1 RNA polymerase beta-subunit
            [Wolbachia pipientis], mRNA sequence.
ACCESSION   EY195901
VERSION     EY195901.1  GI:159496452
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 670
            PolyA tail found inside read...SR being truncated to 628
            Potential full insert read...vec-to-vec length: 549
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggta aaggtgtaag actgccattt gacctagtga atgtaaatgg ggatgtgtta
       61 cttaaagcta atgttcgtat tacagcgaga ttagctaaga ggctctacga tgatggatta
      121 agtgaatatg ttataccttt tgactctata tgcggcctat ttcttgcaaa ggatataatc
      181 gatattgaaa gttctgctat gattttatcg gctggtgagc ccataaagcc tgatgatcta
      241 aagaaaatcc agcttttatc tgtaaatgaa atatcagtat tgaacataga taatgtatcc
      301 gttgggccat acatattgaa tatgcttttc ttggaaaagg atatgtctta tcaggatgct
      361 ctacaggaaa tatatagagt cttacgtcct ggcgaagtgc cagtttttga gatagttgag
      421 agttttttcc agaatctttt ctttaattca gagtattacg atttagaaaa agagaca
//