LOCUS EY195899 257 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab16h03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195899 VERSION EY195899.1 GI:159496450 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 257) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 449 PolyA tail found inside read...SR being truncated to 408 Potential full insert read...vec-to-vec length: 329 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..257 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtg gaagggagag aagaggaggg ccaaacggaa gttggcgcag aaacgaaaat 61 aagccccact aattatgacg aagaggcggt agtagagaac aacaaagaaa agagcgggac 121 tgctggggac gagcacgcga agggggaact ttgagaatgc gaacacccct ggttgtattt 181 cgatcttttt cgggtaattc gatatttatt ctgttctgtc atcttttggc ataaacaaat 241 agaagccaaa aacagta //