LOCUS       EY195893                 528 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab16g04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_508841.1| Actin ACT-4 (41.8 kD) (act-4)
            [Caenorhabditis elegans] sp|P10986|ACT4_CAEEL ACTIN 4 pir|S27135
            actin 4 - Caenorhabditis elegans emb|CAA34720.1| actin
            [Caenorhabditis elegans] gb|AAB04575.1| Actin protein 4, isoform a
            [Caenorhabd, mRNA sequence.
ACCESSION   EY195893
VERSION     EY195893.1  GI:159496444
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 806 was extended to value 807 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 702
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat caccgtcgga aacgagcgct tccgttgtcc cgaggccctc ttccagccct
       61 ctttcctggg catggagtcg gccggcatcc acgagacctc gtacaactcg atcatgaagt
      121 gcgacattga catccgtaag gacctgtacg cgaacaccgt gctctccggc ggcaccacca
      181 tgtacccggg aatcgctgac cgcatgcaga aggagatcac cgcgctggcg ccttccacca
      241 tgaagatcaa gatcatcgcg ccgcctgagc gcaagtactc cgtctggatc ggtggctcca
      301 tcctggcccc gctctctacc ttccagcaga tgtggatctc aaagcaggag tacgacgagt
      361 cgggtccctc gattgttcac cgcaaatgct tctaagaagg gaaagacaac ggcaaatgac
      421 aaagacggac acgaaacaat atctctcttt tttcgtttaa ataagatgtg gtcatggatc
      481 aatcgtttaa aatctgaata aatatggacc aataaaaaaa aaaaaaaa
//