LOCUS       EY195889                 334 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab16f12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195889
VERSION     EY195889.1  GI:159496413
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 531
            PolyA tail found inside read...SR being truncated to 488
            Potential full insert read...vec-to-vec length: 406
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcag taagcggagg aaaagaaact aacaaggatt cccgtagtaa cggcgagtga
       61 actgggaaga gtccagcgct gaatcttggt gcctctggcg ccaagaggtg tagcgtacag
      121 actgcgagtt tgggctggcg cgtcagttca agtctccctt gacaggggtc cctcagaagg
      181 tgcaaggcct gtccaacggg tgcagacagt ccattctcgc gtgtcttgga gtcgggttgt
      241 ttgggatcgc agcccaaaga aggtggtaaa cttcatctaa gactgaatat gactacgaga
      301 ccgatagcga acaagtactg tgaaggaaag tcga
//