LOCUS       EY195888                 473 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab16f11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAK92169.1|AF400197_1 ribosomal protein L35A
            [Spodoptera frugiperda], mRNA sequence.
ACCESSION   EY195888
VERSION     EY195888.1  GI:159496412
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 662
            PolyA tail found inside read...SR being truncated to 624
            Potential full insert read...vec-to-vec length: 545
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac gcccaggaac agaaagcccc tcgtcgccgc gcgggaccga aacctgcgcg
       61 cagactttac gtcaaagcag tgttctccgg tttcaaacgc ggccaccgca accaacacga
      121 gaacacatcg ctgctaaagg tggatggagt gcacgacaag gacgaagcga agttctacct
      181 tgggaagcgt gccgtctacg tctacaaggg gcacaagaag gtcgcgcgag gcggagggcc
      241 taagacgcgc gtccgctgca tttggggtcg cgtaacacgt gtgcatggaa actccggtgt
      301 ggttcgcgcc aaattccgcc acaatctgcc tccgaccgcg atgggaaagc gcatccgaat
      361 tatgtagtac ccatcgaaca tctgagtgga tttgtcgatg acttcgacat gattcttgtt
      421 actctgctgg ttataaaatt gttaccataa gaaaatttta cagcactttt gta
//