LOCUS EY195872 449 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab16e04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195872 VERSION EY195872.1 GI:159496368 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 449) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 643 PolyA tail found inside read...SR being truncated to 600 Potential full insert read...vec-to-vec length: 521 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..449 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccaggaat gataactggg agcgggctgc ccaaagttgg cgtgcgtgtg tggcaccacg 61 agccgaccaa aatcaagaag ttcgaacagt ccgcagagct caaccagggc ctcggcgggc 121 gcgtgctcgc actctgccag agtccgtgcg gagaattcgt tctggccgcc aatcaggacg 181 aagtagtccg aatttggcat gtttggaagg tggacgagtc gatgcgtaaa caagcagcgg 241 aaagcgtgac ggcaaatgcc cgtaaacaac tgcactggaa tggcaagaat gcgcagacgg 301 gaaacgtcat acgataactc tcaacctttt atttaatttt gtgtgttacc tatactatta 361 gtctcattca aactcgcaca ccgataaacc gatatctttt ttcgcttcgc atttaatcgt 421 tgctttttaa aaaaatgcgt acatattta //