LOCUS EY195871 262 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab16e02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|XP_130877.1| similar to GMP synthase [glutamine-hydrolyzing] (Glutamine amidotransferase) (GMP synthetase) [Mus musculus], mRNA sequence. ACCESSION EY195871 VERSION EY195871.1 GI:159496367 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 262) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 821 was extended to value 822 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 457 PolyA tail found inside read...SR being truncated to 414 Potential full insert read...vec-to-vec length: 334 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..262 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac gggccaggca gcacttccgg ggcgagatat tcccctggag acaatccacg 61 aaatggttcg acgcatcgaa acggaaatgc ccacaatctc gcgtgtaatg cttgatctga 121 catcgaaacc tcccgggaca actgaatggg aatgatctat gctctagagt gttttatgaa 181 tattggagta aaatgttgtt tcgctgtcga atgatcgttt tgtttcgttt caacgacttg 241 ttttgataaa tttgtgatta ga //