LOCUS       EY195870                 587 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab16e01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_250697.1| maleylacetoacetate isomerase
            [Pseudomonas aeruginosa PA01] sp|P57109|MAAI_PSEAE
            Maleylacetoacetate isomerase (MAAI) pir|D83394 maleylacetoacetate
            isomerase PA2007 [imported] - Pseudomonas aeruginosa (strain PAO1)
            gb|AAG05395.1|AE, mRNA sequence.
ACCESSION   EY195870
VERSION     EY195870.1  GI:159496338
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 612 (,)
            original QR value of 797 was extended to value 798 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 761
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa gaaaaattcc ccggtcacaa aactctgctc cccggcaccg ccgaagaccg
       61 cgcccaaatc cgcgcgctcg cactccagat catcgtcaac actcagcccc tgcagaacct
      121 tgcagtgctg gagcatttgt ccagtgattc tgacgtaaag aaggaatggg cccgccattg
      181 gatccaaact tcgtttcgga agttggagaa ggtattggcc aaaactgcag gcaaatattc
      241 atacaggaat gagccgagtc tgatcgacgt ttgtattccc ccgcaagtgt ataatgcgaa
      301 gaggtttggt gtggaaatgt ctcaatttcc gactattgga cgactggatg ctactctcgc
      361 cgagatacca gcattcagac tcggagattg ttaccaccag ccagacacgc cagaacagga
      421 gcgcaaaatt gattaaaact ctgcggaagg agacaacatg gttgtgtggg tgaacgaagg
      481 aggtgtctac ctaactccgg acattaatcc tggaggctaa attattctca gattcagata
      541 ttatttacac acttaaaaac aatattccta tgaaaaaaaa aaaaaaa
//