LOCUS       EY195868                 322 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab16d11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195868
VERSION     EY195868.1  GI:159496336
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 785 was extended to value 786 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 515
            PolyA tail found inside read...SR being truncated to 474
            Potential full insert read...vec-to-vec length: 394
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg acgaggaatc gcgagggacg tacgacaaga gcatcttgag acgcatatcc
       61 gtggcgcttt cgttgcgcaa atggcgcacg gaaaatgctt ccgagaaaaa cggaaattcg
      121 tcttcggttg atcgaattgc acggccctcc acggcctccc gtctcaacat atcgtcgccc
      181 atcaaaaggg aaatgggcga attccgcaac ccaaccgccg aagtgaccgg tcggatgtgg
      241 gaagacagac tgttccgatg gatagcggtc ggatttttca cattttgcct ctcctattac
      301 accattgtgc ggtttgtggc ca
//