LOCUS EY195866 212 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab16d09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195866 VERSION EY195866.1 GI:159496334 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 212) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 828 was extended to value 828 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 407 PolyA tail found inside read...SR being truncated to 364 Potential full insert read...vec-to-vec length: 284 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..212 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggata aagcgaaggc tagtggtgga tatttgaaat ggcagatgga tgtttggatc 61 tttttatgac gcggcaaatt tgacaatccg tttttttcat ccatttctcg acttatatta 121 aataaaaatg aaggagagtc gggttgccgt cgtctttcca actcaaatcc tgtattttcc 181 tctcggctaa cgtgataaaa aatattgctc ga //