LOCUS EY195859 362 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab16d01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195859 VERSION EY195859.1 GI:159496299 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 362) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 788 was extended to value 789 (,) This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 558 PolyA tail found inside read...SR being truncated to 514 Potential full insert read...vec-to-vec length: 434 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..362 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggat ggataaaaaa aaaaaaaaaa aaaaaataaa taattccagg aggcaaacgg 61 gggacagcgc ttggctggaa tgcaaactct cttcggattc gctgtttggc cgggcttgtt 121 gagagctcgt agggcgatgc aaaatgaccc gaatcttccc cccgtcttcc actatccgtc 181 acgtgaagag cttaacgagg gacggctgaa cttgaccatc aaaagctgtg tcatgaatat 241 gcttcacgat gcggagtatg aaatccagga gggattcgaa ttcttcatcc ttccgtaaaa 301 tgtggtctat atgtgttttt gatactcatt tgttacgatc tgaaaataaa cagactggtt 361 ga //