LOCUS EY195843 308 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab16b07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|XP_133889.1| similar to hypothetical protein MGC11690 [Mus musculus], mRNA sequence. ACCESSION EY195843 VERSION EY195843.1 GI:159496226 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 308) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 759 was extended to value 760 (,) This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 505 PolyA tail found inside read...SR being truncated to 460 Potential full insert read...vec-to-vec length: 380 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..308 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga gtaaaaggaa cgaggaattg ctctccaacc aaatgcttgt cggcattccc 61 gaagtggatc tgggcatcga gacaagaatg aagaacattg aggaaacgga acaaaagaag 121 gtgcagctgc tgaaaagggg ttcgatcccg cgaatgttcg acgaatggca ccccccgttg 181 ccgtcggaag cggcaaaaag atgaacaact ctcgtgttat gggattcaac cctgaaataa 241 aaactccatt atttccactt ttgtccaaaa taagtatttt atcgagtgat taaacgaaca 301 aaaatgga //