LOCUS       EY195839                 470 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab16b03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195839
VERSION     EY195839.1  GI:159496222
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 807 was extended to value 807 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 702
            PolyA tail found inside read...SR being truncated to 621
            Potential full insert read...vec-to-vec length: 542
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga ccagcgtcta tcgcaccaac ccattcttct cgctgagaag ctacatgcag
       61 gacaagtact accacttctc gcaccgctac aagcgcaccc aattccaccg acactactac
      121 cacggtcaag tcgagaacta cccgtacaga tggaacttcg tccccaaggg caccgccacc
      181 ggccagttct attatgccag acgagtccaa ttcgatcccg tccagtatgg gctgctgcgc
      241 tattatgacc cctaccgtcg ctactacgat ccgtaccgcg actcgtacat cgaattgtac
      301 aactacagac ccaaactgat ggacagcctc tattgaagtg cccagaaccg aatgttagaa
      361 agatggatgg aatggatttg aacgcgataa acagaagcca ttgtggatgg gaactcctct
      421 gtttgtttgt tcagcgaacc ctcgattgag caaagcgatt tgggatacta
//