LOCUS       EY195832                 577 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab16a07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_508841.1| Actin ACT-4 (41.8 kD) (act-4)
            [Caenorhabditis elegans] sp|P10986|ACT4_CAEEL ACTIN 4 pir|S27135
            actin 4 - Caenorhabditis elegans emb|CAA34720.1| actin
            [Caenorhabditis elegans] gb|AAB04575.1| Actin protein 4, isoform a
            [Caenorhabd, mRNA sequence.
ACCESSION   EY195832
VERSION     EY195832.1  GI:159496187
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 795
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggacc gccgagcgtg agatcgtccg cgacataaag gagaagctct gctacgtggc
       61 acttgacttt gagcaggaga tggccaccgc cgcctcctct tcgtcgctgg agaagagcta
      121 cgagttgccc gacggacagg tgatcaccgt cggaaacgag cgcttccgtt gtcccgaggc
      181 cctcttccag ccctctttcc tgggcatgga gtcggccggc atccacgaga cctcgtacaa
      241 ctcgatcatg aagtgcgaca ttgacatccg taaggacctg tacgcgaaca ccgtgctctc
      301 cggcggcacc accatgtacc cgggaatcgc tgaccgcatg cagaaggaga tcaccgcgct
      361 ggcgccttcc accatgaaga tcaagatcat cgcgccgcct gagcgcaagt actccgtctg
      421 gatcggtggc tccatcctgg cctcgctctc taccttccag cagatgtgga tctcaaagca
      481 ggagtacgac gagtcgggtc cctcgattgt tcaccgcaaa tgcttctaag aagggaaaga
      541 caacggcaaa tgacaaagac ggacacgaaa caatatc
//