LOCUS       EY195825                 458 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab11h11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to emb|CAB58171.1| troponin-like protein [Anisakis
            simplex], mRNA sequence.
ACCESSION   EY195825
VERSION     EY195825.1  GI:159496525
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 790 was extended to value 791 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 652
            PolyA tail found inside read...SR being truncated to 610
            Potential full insert read...vec-to-vec length: 530
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggatc cgcaagtttg acgctgacgg ctccggcaag ctggagttcg acgagttctg
       61 cgctctggtg tacactgtgg ccaacacggt ggacaaggac acgttgcgca aggagctgag
      121 ggaggcgttc cgcctgttcg ataaggaggg caacggctac atctctcgcc ccacgctgaa
      181 gggcctgctg aaagagatcg cgcccgacct cagcgacaag gacttggacg cagcggtgga
      241 cgagattgac gaggacggca gcggaaaaat cgagttcgag gagttctggg agctgatggc
      301 cggagagacg gattgagctc tgggagcggg acgagacgga acacacagga atggggaata
      361 atgcgccggc attattattt ggcaaacaat ttgtggtgct cgttcaatcc aatttatttt
      421 gattgtcggt gaaaacaata aacacgcggc cattagca
//