LOCUS       EY195823                 353 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab11h09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195823
VERSION     EY195823.1  GI:159496523
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 823
            PolyA tail found inside read...SR being truncated to 504
            Potential full insert read...vec-to-vec length: 425
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac atatatatct gatgcaattg caaaagaatt aattgaacat ttgcgtcgat
       61 gcgacctaca gattgttagg cgttccgaac tacttaattc cagctcaagg aatgactgtc
      121 aaggagaagt tgaagcgaaa gccgctccac gaatattctg atggcacaga ccgcgaatga
      181 ggacagatag acctgcgaag tgcactatcc ctttgagaca acacatacaa ccaattccca
      241 tttgccgccc tccatctcca actgtgtgat tttaacacgc aaatttccaa aaatcaattt
      301 cgctatcttt taactcaaat ttttgaaaac acacaataaa atatatagaa tta
//