LOCUS       EY195809                 545 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab11g06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_493377.1| Valyl tRNA Synthetase VRS-2 (118.9 kD)
            (vrs-2) [Caenorhabditis elegans] emb|CAB60428.1| C. elegans VRS-2
            protein (corresponding sequence Y87G2A.5) [Caenorhabditis elegans],
            mRNA sequence.
ACCESSION   EY195809
VERSION     EY195809.1  GI:159496509
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 808 was extended to value 809 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 723
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggca cttcggccat acaccatttc tggatttacg agttttgcga cgtctacttg
       61 gaatccgtga agccattgtt taatgcagat gcggagaatt tgagggcaaa tttcgttcgc
      121 cacattctgc accattgtgt ggacactgtt ctccgtttaa ttgcgcccat catgccgttt
      181 atttcggagg aactttggca acggttgcca aaacggaata gcgaatgcgc caaaagcatt
      241 tgtgtggctg attatccaga cactgcaaag tattcctatc gtgacgagga ggtggaattg
      301 cgtttcactg aactacgcga ggctgctctg gcaaagtcgg gcaagcagcc gaagaaaaat
      361 ggagggcagc taaacgacaa cagcacgatg ttcgagaacg gtggagacaa aaatgatgaa
      421 atgaattgac ctacttcacc catctggtcc gttggctggc tggctgtttg ttctttgttg
      481 ttaccattat ttgctgtgaa tttggattta ttgttaaaat taatttaaca aaaaaaaaaa
      541 aaaaa
//