LOCUS       EY195806                 505 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab11g03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195806
VERSION     EY195806.1  GI:159496506
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 815 was extended to value 816 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 698
            PolyA tail found inside read...SR being truncated to 656
            Potential full insert read...vec-to-vec length: 577
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa gaaggaggag tatggaaagg gcaacggcta cggatcggag cagaagagtt
       61 acggaactga ggcaggacaa caaagctacg ggggtcagag cagcggctac aagcacacgt
      121 acccgcagaa gccaaataat ccgcggtgtg tgtgctgtgc accaaaggtg agctacgcac
      181 agagttacga caacgctcag aagaactacg gtggccgacg gaatgagggt ggctactacg
      241 gtcaaggatc atactcggaa gaggacagtg acgaatacgg cggggagcga tatggacagg
      301 gggagcgaca acagcagaac tacgggagca acgagagcgg cgggcatgga cgcaaacagc
      361 agcgtcacgg atatgggagg cagaaccaat acggtggacg gaaggctgga gggtatggaa
      421 acgaatgaag agagggagag agaggaaccg ttacaattga atgatacgga tgagtgatgt
      481 tgctgaataa acgaacaatt ttcga
//