LOCUS       EY195796                 472 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab11f01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_741664.1| Glioma tumor suppressor candidate
            region gene 2 like (49.7 kD) [Caenorhabditis elegans]
            sp|Q9NEU5|GSR2_CAEEL Hypothetical protein Y39B6A.33 in chromosome V
            pir|T45041 hypothetical protein Y39B6B.o [imported] -
            Caenorhabditis elega, mRNA sequence.
ACCESSION   EY195796
VERSION     EY195796.1  GI:159496496
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 663
            PolyA tail found inside read...SR being truncated to 624
            Potential full insert read...vec-to-vec length: 544
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga aaaagatgct ggggacaaga agccagcaac tgcaaaactt gaagtccctg
       61 aacagggaag tgtccggcga gttggaggcc cacaaatctc tcctccgcca acgtcgtgca
      121 aaaaggactc tggggcagct gacacagcgg aagagactgg gccgaggcaa attcgaaccg
      181 tttcaagagc cagtcctcaa cgtcgacgaa ttgccctcaa atttgcgaca gttgagcgta
      241 cagggcaatg tctttctgga gcgcctcaag agtcttcagc agcggaatat tctgccgatt
      301 ggcggggacc gacagcaggc aaagaagtcc cggctgaagg tcaagtttgt ggagaagcgc
      361 agcgcaaagg gagtgagcga gacctctcgg gtgatctgag tccggcactg cactgtttgt
      421 tgttgatttg gttttgctga tccagtatca gagaaatgaa gccaattgct ga
//