LOCUS       EY195795                 449 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab11e11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_497601.1| EF hand calcium binding protein family
            member, possibly N-myristoylated [Caenorhabditis elegans]
            gb|AAF60872.2| Hypothetical protein Y71H2AL.1 [Caenorhabditis
            elegans], mRNA sequence.
ACCESSION   EY195795
VERSION     EY195795.1  GI:159496495
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 802 was extended to value 803 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 643
            PolyA tail found inside read...SR being truncated to 600
            Potential full insert read...vec-to-vec length: 521
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga cgtgttcttc gtggatgcgg aggtctccgc cgagagacgg gtgtacttcc
       61 gcgacttcgt caaagtgctc tcgcacttcc ggcccgtcaa caagaacagt ccgcacgcgt
      121 ggaacagtcg tgaggcgaag ctgaaatttg ccttcacgat gtacgacatc aaccgttcgg
      181 gcacaatcac caaggacgag ttcaccgaga tactggtgcg gatgatcggt gcgaatgtgt
      241 ccaaggagaa ggtggactcg atcgccgaac gcacaatgcg ggaggcggac cgcgactcgg
      301 acggctccat cacattccag gagttttgcc gagcaatgga gaagacagac attgaggaga
      361 agatgagctt ccagttcctg tcctgaagac cacaattgga atgtattccg cgcactcaat
      421 tggctgattc aataaaatgc ctcatcgta
//