LOCUS EY195791 219 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab11e06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_005893.1| MAD, mothers against decapentaplegic homolog 3; MAD (mothers against decapentaplegic, Drosophila) homolog 3; Mothers against decapentaplegic, drosophila, homolog of, 3 [Homo sapiens] ref|NP_037227.1| MAD homolog 3; MAD (mothers agai, mRNA sequence. ACCESSION EY195791 VERSION EY195791.1 GI:159496491 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 219) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 839 was extended to value 840 (,) This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 415 PolyA tail found inside read...SR being truncated to 370 Potential full insert read...vec-to-vec length: 291 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..219 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtt tcgtcaaagg ctggggccag cagtatcgac gacaaacaat aacatcgtgc 61 ccaatgtgga tagagctgca gtttccacgg ccgttgcaaa tgctcgacga agtgctgatg 121 cagtgcgcca gcgaaatagg aatggaggag gtgcacagtt tctcgtagga atggaaaatg 181 gattatccaa ttgcagcgcc gaataaacaa ttcatttca //