LOCUS EY195786 276 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab11e01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195786 VERSION EY195786.1 GI:159496486 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 276) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 153 original value before phred recall for SR was 473 PolyA tail found inside read...SR being truncated to 430 Potential full insert read...vec-to-vec length: 348 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..276 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggaac gaggagaaac ttgtgcagct gctggacatg gtaatttcga ctcgacccaa 61 agacaacagc attgcttcgt tgttcgcgtg cgatgtccac aaagaccgca ttttggagtg 121 caaacgagat gcacgctgtc tggagcgaat gcggagtgtg tggacggcaa aatgcagatg 181 ccaggggaac gaccattttt gcgcgtcctg tttacttttc attcacaatt tcgaactgct 241 ttgcgcattt gcagtgaaaa gacttgtggt tggtta //