LOCUS       EY195781                 244 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab11d06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to pir|I51681 poly(A) polymerase - African clawed frog
            gb|AAA64708.1| poly(A) polymerase, mRNA sequence.
ACCESSION   EY195781
VERSION     EY195781.1  GI:159496481
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 823 was extended to value 824 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 438
            PolyA tail found inside read...SR being truncated to 395
            Potential full insert read...vec-to-vec length: 316
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtt ggtggaaagt tgttcacctc tggttcctac agactcggcg tacacactcc
       61 aggagctgat attgattcat tatgtgttgt gccaagacat atcgacagat ccgatttctt
      121 cacatctttt taccagctac tcaaagaggt tgtttaactt gaccactcat ttgaaatgaa
      181 cataagaaag aactcaaaaa tttgaaaatt ggcaatgatg ttttataaat ctacactttg
      241 aaga
//