LOCUS       EY195777                 458 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab11c12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to emb|CAB58171.1| troponin-like protein [Anisakis
            simplex], mRNA sequence.
ACCESSION   EY195777
VERSION     EY195777.1  GI:159496477
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 782 was extended to value 783 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 655
            PolyA tail found inside read...SR being truncated to 612
            Potential full insert read...vec-to-vec length: 530
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggctg atccgcaagt ttgacgctga cggctccggc aagctggagt tcgacgagtt
       61 ctgcgctctg gtgtacactg tggccaacac ggtggacaag gacacgttgc gcaaggagct
      121 gagggaggcg ttccgcctgt tcgataagga gggcaacggc tacatctctc gccccacgct
      181 gaagggcctg ctgaaagaga tcgcgcccga cctcagcgac aaggacttgg acgcagcggt
      241 ggacgagatt gacgaggacg gcagcggaaa aatcgagttc gaggagttct gggagctgat
      301 ggccggagag acggattgag ctctgggagc gggacgagac ggaacacaca ggaatgggga
      361 ataatgcgcc ggcattatta tttggcaaac aatttgtggt gctcgttcaa tccaatttat
      421 tttgattgtc ggtgaaaaca ataaacacgc ggccatca
//