LOCUS EY195775 387 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab11c10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_492368.1| Intramembrane cleaving protease like (73.7 kD) [Caenorhabditis elegans] pir|T19783 hypothetical protein C36B1.12 - Caenorhabditis elegans emb|CAB02277.1| Hypothetical protein C36B1.12 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY195775 VERSION EY195775.1 GI:159496475 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 387) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 584 PolyA tail found inside read...SR being truncated to 538 Potential full insert read...vec-to-vec length: 459 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..387 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtga tcgcattctg tttctatgtg gacgtggcca gacgcagccg gttctacaca 61 tacgggtcaa tttcattaat cggttacacc ctgggcatgg ccagcacctt tttggcactg 121 ctgcttatgc agtcggggca accggcgctg atctatctcg ttccgttcac cctgatccca 181 atcgcagtga aggcattttt gttcgattca agcggcacat tgacgaaaat gtggcgaggt 241 gagatctgat gcatttgatg tgtttaaaaa aaccaaatcg aactgcttga attattattg 301 atttatttta ctaattgatt attgtatgca ttctgttttc cattgaatcg atttacgcgg 361 atgaatgaat aaagtgaact ttcttta //