LOCUS EY195769 346 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab11c04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195769 VERSION EY195769.1 GI:159496469 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 346) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 824 was extended to value 824 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 541 PolyA tail found inside read...SR being truncated to 497 Potential full insert read...vec-to-vec length: 418 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..346 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa cggactgaag aagtgctgga gctgcgattc attgacttcc atagtggaaa 61 cctgaaatac gcgctgattc gagattccgt tgatcacaaa gcgatctcat gccagcgcat 121 tgaatttgac aagatgacca caactgagct gatcagggag acgattgctt gactgacttt 181 gacttgatgt attgatctga ccacataacc tgatgatgtg atgacaccca gaacatttgc 241 atttgcatac ttttatttgc ttatctcaaa cggctatata atgtttccat cacttctctt 301 gttaacaaac tcaaggagtt ctaatcggaa taaacggcag atttta //