LOCUS EY195756 235 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab11a09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195756 VERSION EY195756.1 GI:159496456 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 235) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 809 was extended to value 809 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 427 PolyA tail found inside read...SR being truncated to 386 Potential full insert read...vec-to-vec length: 307 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..235 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggctg taccaaaatc cgattacaat atacctactt aattttaatt tcccttcaac 61 cccactccac tgatgactga ctggtgatgc gaatgcgaaa tatattattg tagcttataa 121 gattgaaaac cactttaatt tgttagttcc accactctct attttcaatt ttatctaaag 181 attgaatagt tgtatcactg attattttaa tggatggata aaggacgtag ttcga //