LOCUS       EY195516                 300 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab23c01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_003582.2| cullin 2 [Homo sapiens]
            sp|Q13617|CUL2_HUMAN Cullin homolog 2 (CUL-2)
            gb|AAD23581.1|AF126404_1 cullin 2 [Homo sapiens]
            gb|AAH09591.1|AAH09591 Unknown (protein for MGC:14666) [Homo
            sapiens], mRNA sequence.
ACCESSION   EY195516
VERSION     EY195516.1  GI:159498035
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 493
            PolyA tail found inside read...SR being truncated to 451
            Potential full insert read...vec-to-vec length: 372
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa tgcgcaatag tgcgaattat gaaggcgcgg agggtgatga agcatggagc
       61 tttggttgaa gaagtgataa agcaatccca gtcgaggttc atacccgatg ttcagttcat
      121 caagaagaat atagaagcat taatcgacaa atattatttg cagcgcaccg accaggcaga
      181 tgaatacgaa tacatttctt agcattttaa taatcagttc ttccttcaac ttttcaaaca
      241 aagattttac caatttgccc gagatgtatt tacaaacgcc aaaaaaattt agtcgcacga
//