LOCUS EY195515 229 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab23b12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195515 VERSION EY195515.1 GI:159498034 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 229) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 147 original value before phred recall for SR was 449 PolyA tail found inside read...SR being truncated to 380 Potential full insert read...vec-to-vec length: 301 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..229 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtc gctgaattcc cgttcgaaaa cgactcgctg gccgacgaat tttgcgttga 61 attggtcgct caactggatt gccttcgccg gttagagcct tctctgctga taagaaggca 121 attgatggaa gtctgtgcaa attttgccaa tgcaaacacc gatttggcca ctttttgaat 181 ttattgttga gatttgttca atgagatcaa aacagtcccg tttctcgga //