LOCUS       EY195512                 386 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab23b09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAO43307.1| putative polyubiquitin [Arabidopsis
            thaliana], mRNA sequence.
ACCESSION   EY195512
VERSION     EY195512.1  GI:159498031
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 578
            PolyA tail found inside read...SR being truncated to 537
            Potential full insert read...vec-to-vec length: 458
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcga agtggaggga tcggacacca tcgagaatgt gaaagcgaag atccaggaca
       61 aggaaggcat tcccccggac cagcagcgtc tgatcttcgc cggaaagcag ctcgaggacg
      121 gccgcaccct tgcggactac aacatccaga aggagtccac cctccatctg gtgttgcgtc
      181 tccgcggtgg ttccgcatga gtgggagaac gcaacgagga atatgacgtc ttgagatgtg
      241 gacccaactt attcctaatt ccatttcatt cgatgatcaa gcaaaacaat ccacacatgt
      301 tccgatatga cacaacgtct tcatccatct tatgttttcg atatgtaatt tcaaaaacct
      361 gcggaacgat agaacatctc agttga
//