LOCUS       EY195510                 497 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab23b07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195510
VERSION     EY195510.1  GI:159498010
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 690
            PolyA tail found inside read...SR being truncated to 648
            Potential full insert read...vec-to-vec length: 569
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc aaaaagtgtg gccatctcga tttcgaaccc agtggccaaa atgggcagtg
       61 tccaagtgga gatggcctct gttgagcagc cgaagaagat cgagtacatc aacccgttgt
      121 tggctgagaa gagcaacggg gaagtgcagg actctgacgc ggcaaatggg aagacgccaa
      181 cgcggcggca caacggtgca acgccaaaca acgattgggc ggggattaga ctgccgccgc
      241 cggtaccgcc aaagccgaaa ataaagatca acatcacggc cagcgattgt tgcattcaca
      301 cgagagaaat caaataatga agtacaagac atggtaaata tttgtgtacg catcaaacaa
      361 catcagttgt actaaattgt atagtaactc ttaattcact cagactttta tctattttcc
      421 acccattttc agctataaca cttccacatt acatatttta ttgattattg cctccggata
      481 aatgatgtct gtcttca
//