LOCUS       EY195509                 537 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab23b06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195509
VERSION     EY195509.1  GI:159498009
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 828 was extended to value 828 (,)
            putative full insert read: vector-to-vector length is 620 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 768
            PolyA tail found inside read...SR being truncated to 687
            Potential full insert read...vec-to-vec length: 609
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcaa ggacagcggc aacaactcca ccgagctgca ggacgtactt gcgcagttcc
       61 aacagaacct gactggcttc tccaacacca gccaatacaa ctcgttcaaa aactccatca
      121 tcaactgcat caatggacta atcggcaata acccgaccgt ggaggactgc tgcatctact
      181 acatcaacgg attataccca tccaacgggg gcagccaaac cctcgccatg cgcaaaacgt
      241 tcctcgcgac gccgttcttc acctgttaca cgcaggagac tacagtgagc atccagatcg
      301 cgactattag cgtcttctgt ggctgcaatt gaggcgcaag acagaagaga gtggcgtcca
      361 acggattcct ttctcgagga agtcaccacg tgttgagccg caccttttct ctccagcact
      421 ttcctgtgca tagtcctaaa agaggcaaga catcgccctt tgacccaaaa cgacagaaca
      481 ccgccgcttt ttgtagtttt cttacacttt ccttgtgaat aaatggtgcg tgagtta
//