LOCUS       EY195495                 386 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab23a03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_508711.1| Vacuolar proton H+ ATPase VHA-12 (54.8
            kD) (vha-12) [Caenorhabditis elegans] sp|Q19626|VATB_CAEEL Probable
            vacuolar ATP synthase subunit B (V-ATPase B subunit) (Vacuolar
            proton pump B subunit) pir|T34226 hypothetical protein F20B6, mRNA
            sequence.
ACCESSION   EY195495
VERSION     EY195495.1  GI:159497976
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 831
            PolyA tail found inside read...SR being truncated to 537
            Potential full insert read...vec-to-vec length: 458
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa ggctgtcgtt ggtgaagagg cactttcttc agatgactta ctctacttgg
       61 agtttttgac aaagtttgaa aagaacttca ttgcgcaggg gaactacgag aaccgaacag
      121 tgtttgagtc gctagacgtg ggctggcaac tgctgcgcat cttccccaga gaaatgctga
      181 agcgcattcc tgcaaagacc ctggacaagt actaccctag aggaggtgct acggcggcaa
      241 agaacgagtg attgaaaagc aaataacaaa atttacttta gcgatagaaa tgcatactct
      301 ttatctgtcc atttaaaatt ccctacaata cgactattcc tttgtgatat ttatctaatg
      361 agttgtgaag aataaaatcg aacgga
//