LOCUS       EY195493                 558 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab23a01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_741090.1| Putative protein of bilaterial origin
            [Caenorhabditis elegans] pir|T33672 hypothetical protein T24C4.6 -
            Caenorhabditis elegans gb|AAC68925.1| Hypothetical protein T24C4.6a
            [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY195493
VERSION     EY195493.1  GI:159497974
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 813 was extended to value 813 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 744
            PolyA tail found inside read...SR being truncated to 708
            Potential full insert read...vec-to-vec length: 630
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggatc tgggcatttt caattgcgac atcagctccc aatacaaaaa tataccggcg
       61 gaaaagatat gcggagatca cggcgagaac caaatactca acgcgatgga gatgtatttg
      121 gaaagaccca ggacaatgca atctgtgctc aacgagagct accaattgta cagattcgga
      181 accaatttgc atcgccatgt ggaggcactc catctcgtat tacgagcact ttcgctgcat
      241 ctggccaacg ggaatttaca gattgccggc agtgcgagca tgttttacat catcaggaat
      301 gtacgaatga attggaacac aaagcgccat gtgattctgg ccctgctcga cggcatggag
      361 aaccacattg acgagcaggt gatggtgcgg aactgttgcc tgagtttgtg ccagttcgac
      421 attccgcaag acaccctctt caactacggc cgagtggcaa atttgctcgt gaaagtgctc
      481 gaaacgcacc attcggatgc gctcacgcag agaattgtcg tgtttctgct gaattcgatg
      541 gcttgccatg tggaagca
//