LOCUS       EY195490                 370 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22h10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195490
VERSION     EY195490.1  GI:159497954
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 770 was extended to value 771 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 780
            PolyA tail found inside read...SR being truncated to 523
            Potential full insert read...vec-to-vec length: 442
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc atacaacgga ggggccggtt ggtatggtgg gactgcagcc acggccgcaa
       61 atcccccagg acagtggtac aacaacgtgg ccggaagcaa tgccacccaa gcggctcagg
      121 ctgcctcttg gtacggtagc aactggtacg ggaacaccaa cggcgcaatg gcctatgatt
      181 ccaacggcac cgtctagacc aaatcgggaa gtggggactg cgagccggca ctgattgtcc
      241 caaacctgcg attatcactg tccaaccatg tcgctctgtt ttgccccttt tgttgagccc
      301 cgagtttctt gttggctgtt gatgtgtgtc tgttcatttc ggcggttggt tattaaacat
      361 tgagtgggga
//