LOCUS       EY195485                 520 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22h04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_503011.1| ADP-Ribosylation Factor related ARF-6
            (arf-6) [Caenorhabditis elegans] pir|T31519 ADP-ribosylation factor
            Y116A8C.12 [similarity] - Caenorhabditis elegans emb|CAB55153.1| C.
            elegans ARF-6 protein (corresponding sequence Y116A8C.12, mRNA
            sequence.
ACCESSION   EY195485
VERSION     EY195485.1  GI:159497949
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 790 was extended to value 790 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 711
            PolyA tail found inside read...SR being truncated to 671
            Potential full insert read...vec-to-vec length: 592
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaag ctgggccttc tgcggttgcg agacaggaac tggtacgtgc agccgtcttg
       61 cgccaccgcc ggagacgggc tatacgaggg actcacctgg ctgtccacaa actgcaagac
      121 ctgatcccgc ttcacaccct ctcctgcttt ttcctcccct tcctcatcgc caaatcgtgc
      181 tcaacacaat atttatgaaa attaatgtga attcgtttta aagtctctcc acttgctgcg
      241 tatttccgcc ctcccccatt cctcgcatct ttcggaccac attctgctga acaaccgaac
      301 gaacacagat tcgcatttca atattctctt tgtggacaca gattatcgat tggaaacaat
      361 aaattcaact gcacacattt tgtctgccaa gcacttttgt acatttccac cattgaagat
      421 tgaactgtga tcacacaaat tttttatgtc ccacaacaac gtccgttcca tggtttgtat
      481 agtaacattt gcgattacga ttaaaagatt ggattattca
//